論文 - 詳細
| RRC ID | 53582 |
|---|---|
| 著者 | Sham LT, Butler EK, Lebar MD, Kahne D, Bernhardt TG, Ruiz N. |
| タイトル | Bacterial cell wall. MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis. |
| ジャーナル | Science |
| Abstract |
Peptidoglycan (PG) is a polysaccharide matrix that protects bacteria from osmotic lysis. Inhibition of its biogenesis is a proven strategy for killing bacteria with antibiotics. The assembly of PG requires disaccharide-pentapeptide building blocks attached to a polyisoprene lipid carrier called lipid II. Although the stages of lipid II synthesis are known, the identity of the essential flippase that translocates it across the cytoplasmic membrane for PG polymerization is unclear. We developed an assay for lipid II flippase activity and used a chemical genetic strategy to rapidly and specifically block flippase function. We combined these approaches to demonstrate that MurJ is the lipid II flippase in Escherichia coli. |
| 巻・号 | 345(6193) |
| ページ | 220-2 |
| 公開日 | 2014-7-11 |
| DOI | 10.1126/science.1254522 |
| PII | 345/6193/220 |
| PMID | 25013077 |
| PMC | PMC4163187 |
| MeSH | Cell Wall / metabolism* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / antagonists & inhibitors Escherichia coli Proteins / chemistry Escherichia coli Proteins / physiology* Mesylates / pharmacology Models, Molecular Peptidoglycan / biosynthesis* Peptidoglycan / chemistry Phospholipid Transfer Proteins / antagonists & inhibitors Phospholipid Transfer Proteins / chemistry Phospholipid Transfer Proteins / physiology* Protein Conformation Uridine Diphosphate N-Acetylmuramic Acid / analogs & derivatives* Uridine Diphosphate N-Acetylmuramic Acid / metabolism |
| IF | 41.846 |
| 引用数 | 131 |
| リソース情報 | |
| 原核生物(大腸菌) | |