RRC ID 53608
Author Yamamoto K, Nonaka G, Ozawa T, Takumi K, Ishihama A.
Title Induction of the Escherichia coli yijE gene expression by cystine.
Journal Biosci. Biotechnol. Biochem.
Abstract Cystine is formed from two molecules of the cysteine under oxidized conditions, but is reversibly converted to cysteine by reduction. Growth of Escherichia coli is retarded in the presence of excess cystine. Transcriptome analysis showed 11 up-regulated and 26 down-regulated genes upon exposure to excess cystine. The reporter assay confirmed regulation by cystine of the expression of one up-regulated membrane gene, yijE, and two down-regulated membrane genes, yhdT and yihN. In order to identify the as yet unidentified gene encoding cystine efflux transporter, the putative cystine efflux candidate, yijE gene, was over-expressed. Expression of the yijE gene suppressed the slow growth of E. coli in the presence of high concentration of extracellular cystine. In good agreement, the knock-out of yijE gene increased the sensibility to cystine. These observations altogether imply that the yijE gene is involved in response to cystine in E. coli.
Volume 79(2)
Pages 218-22
Published 2015
DOI 10.1080/09168451.2014.972328
PMID 25346166
MeSH Cystine / pharmacology* Escherichia coli / drug effects Escherichia coli / genetics* Escherichia coli Proteins / genetics* Gene Expression Profiling Gene Knockout Techniques Genes, Reporter / genetics Membrane Proteins / genetics* Transcriptional Activation / drug effects*
IF 1.297
Times Cited 3
Prokaryotes E. coli