Reference - Detail
| RRC ID | 53614 |
|---|---|
| Author | Lu S, Fan SB, Yang B, Li YX, Meng JM, Wu L, Li P, Zhang K, Zhang MJ, Fu Y, Luo J, Sun RX, He SM, Dong MQ. |
| Title | Mapping native disulfide bonds at a proteome scale. |
| Journal | Nat Methods |
| Abstract |
We developed a high-throughput mass spectrometry method, pLink-SS (http://pfind.ict.ac.cn/software/pLink/2014/pLink-SS.html), for precise identification of disulfide-linked peptides. Using pLink-SS, we mapped all native disulfide bonds of a monoclonal antibody and ten standard proteins. We performed disulfide proteome analyses and identified 199 disulfide bonds in Escherichia coli and 568 in proteins secreted by human endothelial cells. We discovered many regulatory disulfide bonds involving catalytic or metal-binding cysteine residues. |
| Volume | 12(4) |
| Pages | 329-31 |
| Published | 2015-4-1 |
| DOI | 10.1038/nmeth.3283 |
| PII | nmeth.3283 |
| PMID | 25664544 |
| MeSH | Amino Acid Sequence Disulfides / chemistry* Escherichia coli / chemistry Humans Mass Spectrometry* Models, Molecular Molecular Sequence Data Peptide Library Proteome / chemistry* Proteomics / methods* Ribonucleases / chemistry |
| IF | 30.822 |
| Resource | |
| Prokaryotes E. coli | |