RRC ID 53617
Author Ogasawara H, Ohe S, Ishihama A.
Title Role of transcription factor NimR (YeaM) in sensitivity control of Escherichia coli to 2-nitroimidazole.
Journal FEMS Microbiol Lett
Abstract The binding site(s) on the Escherichia coli genome was determined for an uncharacterized AraC/XylS superfamily transcription factor YeaM by using the in vitro genomic SELEX screening system. The only one clear binding target of YeaM was found to locate in the spacer between the divergently transcribed yeaM and yeaN genes. After the phenotype microarray analysis, the major facilitator superfamily transporter YeaN was found to confer E. coli the resistance to 2-nitroimidazole, the antibacterial and antifungal antibiotic, suggesting that YeaN plays a role in 2-nitromidazole efflux. Purified YeaM bound to three sites within this yeaM-yeaN spacer region. Several lines of in vitro and in vivo evidence indicate that YeaM regulates transcription of both the yeaM gene itself and the yeaNO operon. Taken together we propose to rename yeaN to nimT (nitroimidazole transporter) and yeaM to nimR (regulator of nimT).
Volume 362(1)
Pages 1-8
Published 2015-1-1
DOI 10.1093/femsle/fnu013
PII fnu013
PMID 25790494
MeSH Anti-Bacterial Agents / metabolism* Binding Sites DNA, Bacterial / metabolism Drug Resistance, Bacterial* Electrophoretic Mobility Shift Assay Escherichia coli / drug effects* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Profiling Gene Expression Regulation, Bacterial Microarray Analysis Nitroimidazoles / metabolism* Protein Binding Transcription Factors / genetics Transcription Factors / metabolism*
IF 1.987
Times Cited 10
Prokaryotes E. coli