RRC ID 53631
Author Wu H, Tuli L, Bennett GN, San KY.
Title Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli.
Journal Metab. Eng.
Abstract A novel strategy to finely control a large metabolic flux by using a "metabolic transistor" approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The change of the basal level of the essential component, considered as a base current in a transistor, has a large effect on the flux through the major pathway. In this way, the fine-tuning of a large flux can be accomplished. The "metabolic transistor" strategy was applied to control electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichia coli. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy upon manipulation of the biosynthetic pathway of the key participant, ubiquinone-8 (Q8), in an E. coli strain provides an in vivo, genetically tunable means to control the activity of the electron transfer chain and manipulate the production of reduced products while limiting consumption of oxygen to a defined amount.
Volume 28
Pages 159-168
Published 2015-3
DOI 10.1016/j.ymben.2015.01.002
PII S1096-7176(15)00004-X
PMID 25596510
PMC PMC4355220
MeSH Electron Transport Chain Complex Proteins* / genetics Electron Transport Chain Complex Proteins* / metabolism Escherichia coli* / enzymology Escherichia coli* / genetics Escherichia coli Proteins* / genetics Escherichia coli Proteins* / metabolism Lactic Acid / metabolism Oxygen Consumption / genetics* Ubiquinone / genetics Ubiquinone / metabolism
IF 7.808
Prokaryotes E. coli