Reference - Detail
| RRC ID | 53632 |
|---|---|
| Author | Honjo H, Tsuruno K, Tatsuke T, Sato M, Hanai T. |
| Title | Dual synthetic pathway for 3-hydroxypropionic acid production in engineered Escherichia coli. |
| Journal | J Biosci Bioeng |
| Abstract |
3-Hydroxypropionic acid (3-HP) is an important platform C3 chemical; production of 3-HP in recombinant Escherichia coli by synthetic pathways has been the focus of a lot of research. When glycerol is used as a substrate to produce 3-HP in E. coli, only the ALDH pathway (employing aldehyde dehydrogenase (ALDH) for conversion of 3-hydroxypropionaldehyde (3-HPA) into 3-HP) has been utilized as a synthetic pathway. However, several bacteria (including Klebsiella pneumoniae) are known to have the ability to produce 3-HP by the Pdu pathway (employing the PduP, PduL, and PduW enzymes). Here, we report the production of 3-HP in E. coli by using the Pdu pathway from K. pneumoniae as a synthetic pathway. Moreover, a strain harboring a dual synthetic pathways (ALDH and Pdu) exhibited a 70% increase in 3-HP titer compared to one harboring the ALDH pathway alone (56.1 ± 0.736 mM and 33.1 ± 0.920 mM, respectively). To our knowledge, this is the first report of 3-HP production by E. coli harboring the Pdu pathway, with the dual synthetic pathway showing the highest yield ever reported by batch culture [54.1% (mol/mol)]. |
| Volume | 120(2) |
| Pages | 199-204 |
| Published | 2015-8-1 |
| DOI | 10.1016/j.jbiosc.2014.12.023 |
| PII | S1389-1723(14)00503-9 |
| PMID | 25650075 |
| MeSH | Aldehyde Dehydrogenase / genetics Aldehyde Dehydrogenase / metabolism Batch Cell Culture Techniques Biosynthetic Pathways / genetics* Escherichia coli / genetics* Escherichia coli / metabolism* Glyceraldehyde / analogs & derivatives Glyceraldehyde / metabolism Glycerol / metabolism Klebsiella pneumoniae / enzymology* Klebsiella pneumoniae / genetics* Klebsiella pneumoniae / metabolism Lactic Acid / analogs & derivatives* Lactic Acid / biosynthesis Metabolic Engineering* Propane / metabolism |
| IF | 2.366 |
| Times Cited | 20 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |