RRC ID 53636
著者 Ojima Y, Suparman A, Nguyen MH, Sakka M, Sakka K, Taya M.
タイトル Exopolysaccharide assay in Escherichia coli microcolonies using a cleavable fusion protein of GFP-labeled carbohydrate-binding module.
ジャーナル J Microbiol Methods
Abstract A fused protein composed of a carbohydrate-binding module and green fluorescence protein (GFP) was developed to measure the exopolysaccharides (EPShs) present in Escherichia coli microcolonies. The cleavage of the GFP part of this protein using a site-specific protease allowed for the non-invasive and quantitative evaluation of the EPShs.
巻・号 114
ページ 75-7
公開日 2015-7-1
DOI 10.1016/j.mimet.2015.05.013
PII S0167-7012(15)00152-9
PMID 25978970
MeSH Escherichia coli / chemistry* Escherichia coli / growth & development* Genes, Reporter Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics Polysaccharides, Bacterial / analysis* Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism
IF 1.701
引用数 2
リソース情報
原核生物(大腸菌)