論文 - 詳細
| RRC ID | 53650 |
|---|---|
| 著者 | Shimada T, Takada H, Yamamoto K, Ishihama A. |
| タイトル | Expanded roles of two-component response regulator OmpR in Escherichia coli: genomic SELEX search for novel regulation targets. |
| ジャーナル | Genes Cells |
| Abstract |
The two-component system (TCS) is a sophisticated bacterial signal transduction system for regulation of genome transcription in response to environmental conditions. The EnvZ-OmpR system is one of the well-characterized TCS of Escherichia coli, responding to changes in environmental osmolality. Regulation has largely focused on the differential expression of two porins, OmpF and OmpC, which transport small molecules across the outer membrane. Recently, it has become apparent that OmpR serves a more global regulatory role and regulates additional targets. To identify the entire set of regulatory targets of OmpR, we performed the genomic SELEX screening of OmpR-binding sites along the E. coli genome. As a result, more than 30 novel genes have been identified to be under the direct control of OmpR. One abundant group includes the genes encoding a variety of membrane-associated transporters that mediate uptake or efflux of small molecules, while another group encodes a set of transcription regulators, raising a concept that OmpR is poised to control a diverse set of responses by altering downstream transcriptional regulators. |
| 巻・号 | 20(11) |
| ページ | 915-31 |
| 公開日 | 2015-11-1 |
| DOI | 10.1111/gtc.12282 |
| PMID | 26332955 |
| MeSH | Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism Bacterial Proteins / genetics* Bacterial Proteins / metabolism Base Sequence Escherichia coli / genetics* Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial Genomics Multienzyme Complexes / genetics Multienzyme Complexes / metabolism Phosphorylation Response Elements SELEX Aptamer Technique / methods Signal Transduction / genetics Trans-Activators / genetics* Trans-Activators / metabolism Transcription, Genetic |
| IF | 1.655 |
| 引用数 | 13 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |