| RRC ID |
53655
|
| Author |
Barton NR, Burgard AP, Burk MJ, Crater JS, Osterhout RE, Pharkya P, Steer BA, Sun J, Trawick JD, Van Dien SJ, Yang TH, Yim H.
|
| Title |
An integrated biotechnology platform for developing sustainable chemical processes.
|
| Journal |
J Ind Microbiol Biotechnol
|
| Abstract |
Genomatica has established an integrated computational/experimental metabolic engineering platform to design, create, and optimize novel high performance organisms and bioprocesses. Here we present our platform and its use to develop E. coli strains for production of the industrial chemical 1,4-butanediol (BDO) from sugars. A series of examples are given to demonstrate how a rational approach to strain engineering, including carefully designed diagnostic experiments, provided critical insights about pathway bottlenecks, byproducts, expression balancing, and commercial robustness, leading to a superior BDO production strain and process.
|
| Volume |
42(3)
|
| Pages |
349-60
|
| Published |
2015-3-1
|
| DOI |
10.1007/s10295-014-1541-1
|
| PMID |
25416472
|
| MeSH |
Biotechnology / methods*
Butylene Glycols / metabolism
Carbon Isotopes
Escherichia coli / enzymology
Escherichia coli / genetics
Escherichia coli / metabolism
Fermentation
Green Chemistry Technology*
Metabolic Engineering
Metabolic Networks and Pathways / genetics
Systems Biology
|
| IF |
3.103
|
| Times Cited |
28
|
| Resource |
| Prokaryotes E. coli |
|
