RRC ID 53661
Author Lu S, Fan SB, Yang B, Li YX, Meng JM, Wu L, Li P, Zhang K, Zhang MJ, Fu Y, Luo J, Sun RX, He SM, Dong MQ.
Title Mapping native disulfide bonds at a proteome scale.
Journal Nat Methods
Abstract We developed a high-throughput mass spectrometry method, pLink-SS (http://pfind.ict.ac.cn/software/pLink/2014/pLink-SS.html), for precise identification of disulfide-linked peptides. Using pLink-SS, we mapped all native disulfide bonds of a monoclonal antibody and ten standard proteins. We performed disulfide proteome analyses and identified 199 disulfide bonds in Escherichia coli and 568 in proteins secreted by human endothelial cells. We discovered many regulatory disulfide bonds involving catalytic or metal-binding cysteine residues.
Volume 12(4)
Pages 329-31
Published 2015-4-1
DOI 10.1038/nmeth.3283
PII nmeth.3283
PMID 25664544
MeSH Amino Acid Sequence Disulfides / chemistry* Escherichia coli / chemistry Humans Mass Spectrometry* Models, Molecular Molecular Sequence Data Peptide Library Proteome / chemistry* Proteomics / methods* Ribonucleases / chemistry
IF 30.822
Times Cited 65
Prokaryotes E. coli