論文 - 詳細
RRC ID | 53661 |
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著者 | Lu S, Fan SB, Yang B, Li YX, Meng JM, Wu L, Li P, Zhang K, Zhang MJ, Fu Y, Luo J, Sun RX, He SM, Dong MQ. |
タイトル | Mapping native disulfide bonds at a proteome scale. |
ジャーナル | Nat Methods |
Abstract |
We developed a high-throughput mass spectrometry method, pLink-SS (http://pfind.ict.ac.cn/software/pLink/2014/pLink-SS.html), for precise identification of disulfide-linked peptides. Using pLink-SS, we mapped all native disulfide bonds of a monoclonal antibody and ten standard proteins. We performed disulfide proteome analyses and identified 199 disulfide bonds in Escherichia coli and 568 in proteins secreted by human endothelial cells. We discovered many regulatory disulfide bonds involving catalytic or metal-binding cysteine residues. |
巻・号 | 12(4) |
ページ | 329-31 |
公開日 | 2015-4-1 |
DOI | 10.1038/nmeth.3283 |
PII | nmeth.3283 |
PMID | 25664544 |
MeSH | Amino Acid Sequence Disulfides / chemistry* Escherichia coli / chemistry Humans Mass Spectrometry* Models, Molecular Molecular Sequence Data Peptide Library Proteome / chemistry* Proteomics / methods* Ribonucleases / chemistry |
IF | 30.822 |
引用数 | 65 |
リソース情報 | |
原核生物(大腸菌) |