RRC ID 53678
Author Ishijima S, Uda M, Hirata T, Shibata M, Kitagawa N, Sagami I.
Title Magnesium uptake of Arabidopsis transporters, AtMRS2-10 and AtMRS2-11, expressed in Escherichia coli mutants: Complementation and growth inhibition by aluminum.
Journal Biochim Biophys Acta
Abstract Magnesium (Mg2+) plays a critical role in many physiological processes. Mg2+ transport systems in Salmonella have been well documented, but those in Escherichia coli have not been fully elucidated. We examined the effects of corA, mgtA, yhiD and corC gene deletion on Mg2+ transport in E. coli. We obtained every combination of double, triple and quadruple mutants. The corA and mgtA double mutant required addition of 10 mM Mg2+ to Luria-Bertani (LB) medium for growth, and the corA, mgtA and yhiD triple mutant TM2 required a higher Mg2+ concentration. The Mg2+ requirement of the quadruple mutant was similar to that of TM2. The results demonstrated that either CorA or MgtA is necessary for normal E. coli growth in LB medium and that YhiD plays a role in Mg2+ transport under high Mg2+ growth conditions in E. coli. The Arabidopsis Mg2+ transporters, AtMRS2-10 and AtMRS2-11, were heterologously expressed in TM2 cells. TM2 cells expressing AtMRS2-10 and AtMRS2-11 could grow in LB medium that had been supplemented with 1 mM Mg2+ and without Mg2+ supplementation, respectively, and cell growth was inhibited by 2 mM AlCl3. The results indicated that the growth of TM2 expressing AtMRS2-10 and AtMRS2-11 reflected these AtMRS2 function for Mg2+ and aluminum. The E. coli TM2 cells are useful for functional analysis of Arabidopsis MRS2 proteins.
Volume 1848(6)
Pages 1376-82
Published 2015-6-1
DOI 10.1016/j.bbamem.2015.03.005
PII S0005-2736(15)00076-0
PMID 25772503
MeSH Aluminum / toxicity* Arabidopsis / metabolism* Arabidopsis Proteins / metabolism* Culture Media / pharmacology Escherichia coli / drug effects Escherichia coli / genetics Escherichia coli / growth & development* Genetic Complementation Test* Magnesium / metabolism* Membrane Transport Proteins / metabolism* Mutation Transformation, Genetic / drug effects
IF 3.411
Times Cited 6
Prokaryotes E. coli