論文 - 詳細
| RRC ID | 53682 |
|---|---|
| 著者 | Singh SK, Parveen S, SaiSree L, Reddy M. |
| タイトル | Regulated proteolysis of a cross-link-specific peptidoglycan hydrolase contributes to bacterial morphogenesis. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Bacterial growth and morphogenesis are intimately coupled to expansion of peptidoglycan (PG), an extensively cross-linked macromolecule that forms a protective mesh-like sacculus around the cytoplasmic membrane. Growth of the PG sacculus is a dynamic event requiring the concerted action of hydrolases that cleave the cross-links for insertion of new material and synthases that catalyze cross-link formation; however, the factors that regulate PG expansion during bacterial growth are poorly understood. Here, we show that the PG hydrolase MepS (formerly Spr), which is specific to cleavage of cross-links during PG expansion in Escherichia coli, is modulated by proteolysis. Using combined genetic, molecular, and biochemical approaches, we demonstrate that MepS is rapidly degraded by a proteolytic system comprising an outer membrane lipoprotein of unknown function, NlpI, and a periplasmic protease, Prc (or Tsp). In summary, our results indicate that the NlpI-Prc system contributes to growth and enlargement of the PG sacculus by modulating the cellular levels of the cross-link-cleaving hydrolase MepS. Overall, this study signifies the importance of PG cross-link cleavage and its regulation in bacterial cell wall biogenesis. |
| 巻・号 | 112(35) |
| ページ | 10956-61 |
| 公開日 | 2015-9-1 |
| DOI | 10.1073/pnas.1507760112 |
| PII | 1507760112 |
| PMID | 26283368 |
| PMC | PMC4568209 |
| MeSH | Cross-Linking Reagents / metabolism Escherichia coli / growth & development* Escherichia coli Proteins / metabolism Morphogenesis* N-Acetylmuramoyl-L-alanine Amidase / metabolism* Peptidoglycan / metabolism* Proteolysis |
| IF | 9.412 |
| 引用数 | 34 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |