RRC ID 53683
著者 Levy A, Goren MG, Yosef I, Auster O, Manor M, Amitai G, Edgar R, Qimron U, Sorek R.
タイトル CRISPR adaptation biases explain preference for acquisition of foreign DNA.
ジャーナル Nature
Abstract CRISPR-Cas (clustered, regularly interspaced short palindromic repeats coupled with CRISPR-associated proteins) is a bacterial immunity system that protects against invading phages or plasmids. In the process of CRISPR adaptation, short pieces of DNA ('spacers') are acquired from foreign elements and integrated into the CRISPR array. So far, it has remained a mystery how spacers are preferentially acquired from the foreign DNA while the self chromosome is avoided. Here we show that spacer acquisition is replication-dependent, and that DNA breaks formed at stalled replication forks promote spacer acquisition. Chromosomal hotspots of spacer acquisition were confined by Chi sites, which are sequence octamers highly enriched on the bacterial chromosome, suggesting that these sites limit spacer acquisition from self DNA. We further show that the avoidance of self is mediated by the RecBCD double-stranded DNA break repair complex. Our results suggest that, in Escherichia coli, acquisition of new spacers largely depends on RecBCD-mediated processing of double-stranded DNA breaks occurring primarily at replication forks, and that the preference for foreign DNA is achieved through the higher density of Chi sites on the self chromosome, in combination with the higher number of forks on the foreign DNA. This model explains the strong preference to acquire spacers both from high copy plasmids and from phages.
巻・号 520(7548)
ページ 505-510
公開日 2015-4-23
DOI 10.1038/nature14302
PMID 25874675
PMC PMC4561520
MeSH Adaptation, Physiological* Bacteriophages / genetics* CRISPR-Cas Systems / genetics Clustered Regularly Interspaced Short Palindromic Repeats / genetics* Consensus Sequence / genetics DNA Breaks, Double-Stranded DNA Repair DNA Replication / genetics DNA, Bacterial / genetics* DNA, Viral / genetics* Escherichia coli / genetics* Exodeoxyribonuclease V / metabolism Models, Biological Plasmids / genetics*
IF 42.779
引用数 181
リソース情報
原核生物(大腸菌)