RRC ID 53684
著者 Yosef I, Manor M, Kiro R, Qimron U.
タイトル Temperate and lytic bacteriophages programmed to sensitize and kill antibiotic-resistant bacteria.
ジャーナル Proc Natl Acad Sci U S A
Abstract The increasing threat of pathogen resistance to antibiotics requires the development of novel antimicrobial strategies. Here we present a proof of concept for a genetic strategy that aims to sensitize bacteria to antibiotics and selectively kill antibiotic-resistant bacteria. We use temperate phages to deliver a functional clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) system into the genome of antibiotic-resistant bacteria. The delivered CRISPR-Cas system destroys both antibiotic resistance-conferring plasmids and genetically modified lytic phages. This linkage between antibiotic sensitization and protection from lytic phages is a key feature of the strategy. It allows programming of lytic phages to kill only antibiotic-resistant bacteria while protecting antibiotic-sensitized bacteria. Phages designed according to this strategy may be used on hospital surfaces and hand sanitizers to facilitate replacement of antibiotic-resistant pathogens with sensitive ones.
巻・号 112(23)
ページ 7267-72
公開日 2015-6-9
DOI 10.1073/pnas.1500107112
PII 1500107112
PMID 26060300
PMC PMC4466736
MeSH Bacteria / genetics Bacteria / virology* Bacteriophages / physiology* Clustered Regularly Interspaced Short Palindromic Repeats Drug Resistance, Bacterial* Lysogeny Plasmids
IF 9.412
引用数 154
リソース情報
原核生物(大腸菌)