RRC ID |
53684
|
著者 |
Yosef I, Manor M, Kiro R, Qimron U.
|
タイトル |
Temperate and lytic bacteriophages programmed to sensitize and kill antibiotic-resistant bacteria.
|
ジャーナル |
Proc Natl Acad Sci U S A
|
Abstract |
The increasing threat of pathogen resistance to antibiotics requires the development of novel antimicrobial strategies. Here we present a proof of concept for a genetic strategy that aims to sensitize bacteria to antibiotics and selectively kill antibiotic-resistant bacteria. We use temperate phages to deliver a functional clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) system into the genome of antibiotic-resistant bacteria. The delivered CRISPR-Cas system destroys both antibiotic resistance-conferring plasmids and genetically modified lytic phages. This linkage between antibiotic sensitization and protection from lytic phages is a key feature of the strategy. It allows programming of lytic phages to kill only antibiotic-resistant bacteria while protecting antibiotic-sensitized bacteria. Phages designed according to this strategy may be used on hospital surfaces and hand sanitizers to facilitate replacement of antibiotic-resistant pathogens with sensitive ones.
|
巻・号 |
112(23)
|
ページ |
7267-72
|
公開日 |
2015-6-9
|
DOI |
10.1073/pnas.1500107112
|
PII |
1500107112
|
PMID |
26060300
|
PMC |
PMC4466736
|
MeSH |
Bacteria / genetics
Bacteria / virology*
Bacteriophages / physiology*
Clustered Regularly Interspaced Short Palindromic Repeats
Drug Resistance, Bacterial*
Lysogeny
Plasmids
|
IF |
9.412
|
引用数 |
154
|
リソース情報 |
原核生物(大腸菌) |
|