論文 - 詳細
| RRC ID | 53694 |
|---|---|
| 著者 | Benhalevy D, Bochkareva ES, Biran I, Bibi E. |
| タイトル | Model Uracil-Rich RNAs and Membrane Protein mRNAs Interact Specifically with Cold Shock Proteins in Escherichia coli. |
| ジャーナル | PLoS One |
| Abstract |
Are integral membrane protein-encoding mRNAs (MPRs) different from other mRNAs such as those encoding cytosolic mRNAs (CPRs)? This is implied from the emerging concept that MPRs are specifically recognized and delivered to membrane-bound ribosomes in a translation-independent manner. MPRs might be recognized through uracil-rich segments that encode hydrophobic transmembrane helices. To investigate this hypothesis, we designed DNA sequences encoding model untranslatable transcripts that mimic MPRs or CPRs. By utilizing in vitro-synthesized biotinylated RNAs mixed with Escherichia coli extracts, we identified a highly specific interaction that takes place between transcripts that mimic MPRs and the cold shock proteins CspE and CspC, which are normally expressed under physiological conditions. Co-purification studies with E. coli expressing 6His-tagged CspE or CspC confirmed that the specific interaction occurs in vivo not only with the model uracil-rich untranslatable transcripts but also with endogenous MPRs. Our results suggest that the evolutionarily conserved cold shock proteins may have a role, possibly as promiscuous chaperons, in the biogenesis of MPRs. |
| 巻・号 | 10(7) |
| ページ | e0134413 |
| 公開日 | 2015-1-1 |
| DOI | 10.1371/journal.pone.0134413 |
| PII | PONE-D-15-15350 |
| PMID | 26225847 |
| PMC | PMC4520561 |
| MeSH | Cell Membrane / metabolism Cold Shock Proteins and Peptides / metabolism* Escherichia coli / metabolism* Protein Binding RNA, Bacterial / metabolism* RNA, Messenger / metabolism Uracil / metabolism* |
| IF | 2.74 |
| 引用数 | 9 |
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| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |