論文 - 詳細
| RRC ID | 53709 |
|---|---|
| 著者 | Sandoval JM, Arenas FA, García JA, Díaz-Vásquez WA, Valdivia-González M, Sabotier M, Vásquez CC. |
| タイトル | Escherichia coli 6-phosphogluconate dehydrogenase aids in tellurite resistance by reducing the toxicant in a NADPH-dependent manner. |
| ジャーナル | Microbiol Res |
| Abstract |
Exposure to the tellurium oxyanion tellurite (TeO3(2-)) results in the establishment of an oxidative stress status in most microorganisms. Usually, bacteria growing in the presence of the toxicant turn black because of the reduction of tellurite (Te(4+)) to the less-toxic elemental tellurium (Te(0)). In vitro, at least part of tellurite reduction occurs enzymatically in a nicotinamide dinucleotide-dependent reaction. In this work, we show that TeO3(2-) reduction by crude extracts of Escherichia coli overexpressing the zwf gene (encoding glucose-6-phosphate dehydrogenase) takes place preferentially in the presence of NADPH instead of NADH. The enzyme responsible for toxicant reduction was identified as 6-phosphogluconate dehydrogenase (Gnd). The gnd gene showed a subtle induction at short times after toxicant exposure while strains lacking gnd were more susceptible to the toxicant. These results suggest that both NADPH-generating enzymes from the pentose phosphate shunt may be involved in tellurite detoxification and resistance in E. coli. |
| 巻・号 | 177 |
| ページ | 22-7 |
| 公開日 | 2015-8-1 |
| DOI | 10.1016/j.micres.2015.05.002 |
| PII | S0944-5013(15)00089-0 |
| PMID | 26211962 |
| MeSH | Drug Resistance, Bacterial* Escherichia coli / drug effects Escherichia coli / enzymology* Escherichia coli / metabolism* Inactivation, Metabolic NADP / metabolism* Oxidation-Reduction Phosphogluconate Dehydrogenase / metabolism* Tellurium / metabolism* Tellurium / toxicity |
| IF | 3.97 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |