Reference - Detail
| RRC ID | 53714 |
|---|---|
| Author | Pinto F, Pacheco CC, Oliveira P, Montagud A, Landels A, Couto N, Wright PC, Urchueguía JF, Tamagnini P. |
| Title | Improving a Synechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites. |
| Journal | DNA Res |
| Abstract |
The use of microorganisms as cell factories frequently requires extensive molecular manipulation. Therefore, the identification of genomic neutral sites for the stable integration of ectopic DNA is required to ensure a successful outcome. Here we describe the genome mapping and validation of five neutral sites in the chromosome of Synechocystis sp. PCC 6803, foreseeing the use of this cyanobacterium as a photoautotrophic chassis. To evaluate the neutrality of these loci, insertion/deletion mutants were produced, and to assess their functionality, a synthetic green fluorescent reporter module was introduced. The constructed integrative vectors include a BioBrick-compatible multiple cloning site insulated by transcription terminators, constituting robust cloning interfaces for synthetic biology approaches. Moreover, Synechocystis mutants (chassis) ready to receive purpose-built synthetic modules/circuits are also available. This work presents a systematic approach to map and validate chromosomal neutral sites in cyanobacteria, and that can be extended to other organisms. |
| Volume | 22(6) |
| Pages | 425-37 |
| Published | 2015-12-1 |
| DOI | 10.1093/dnares/dsv024 |
| PII | dsv024 |
| PMID | 26490728 |
| PMC | PMC4675711 |
| MeSH | Autotrophic Processes Chromosome Mapping Cyanobacteria / genetics* Cyanobacteria / growth & development Green Fluorescent Proteins Mutation Synechocystis / genetics* Synthetic Biology / methods |
| IF | 4.009 |
| Times Cited | 19 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |