論文 - 詳細
| RRC ID | 53720 |
|---|---|
| 著者 | Tse L, Kang TM, Yuan J, Mihora D, Becket E, Maslowska KH, Schaaper RM, Miller JH. |
| タイトル | Extreme dNTP pool changes and hypermutability in dcd ndk strains. |
| ジャーナル | Mutat Res |
| Abstract |
Cells lacking deoxycytidine deaminase (DCD) have been shown to have imbalances in the normal dNTP pools that lead to multiple phenotypes, including increased mutagenesis, increased sensitivity to oxidizing agents, and to a number of antibiotics. In particular, there is an increased dCTP pool, often accompanied by a decreased dTTP pool. In the work presented here, we show that double mutants of Escherichia coli lacking both DCD and NDK (nucleoside diphosphate kinase) have even more extreme imbalances of dNTPs than mutants lacking only one or the other of these enzymes. In particular, the dCTP pool rises to very high levels, exceeding even the cellular ATP level by several-fold. This increased level of dCTP, coupled with more modest changes in other dNTPs, results in exceptionally high mutation levels. The high mutation levels are attenuated by the addition of thymidine. The results corroborate the critical importance of controlling DNA precursor levels for promoting genome stability. We also show that the addition of certain exogenous nucleosides can influence replication errors in DCD-proficient strains that are deficient in mismatch repair. |
| 巻・号 | 784-785 |
| ページ | 16-24 |
| 公開日 | 2016-1-1 |
| DOI | 10.1016/j.mrfmmm.2015.12.004 |
| PII | S0027-5107(15)30057-9 |
| PMID | 26789486 |
| PMC | PMC4753124 |
| MeSH | Cytidine Deaminase / genetics* Cytidine Deaminase / metabolism DNA-Directed RNA Polymerases Deoxyribonucleotides / genetics Deoxyribonucleotides / metabolism Escherichia coli / drug effects Escherichia coli / genetics* Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Mutation* Mutation Rate Nucleoside-Diphosphate Kinase / genetics* Nucleoside-Diphosphate Kinase / metabolism Thymidine / pharmacology |
| IF | 2.463 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | |