論文 - 詳細
| RRC ID | 53725 |
|---|---|
| 著者 | Tanaka N, Kanazawa M, Tonosaki K, Yokoyama N, Kuzuyama T, Takahashi Y. |
| タイトル | Novel features of the ISC machinery revealed by characterization of Escherichia coli mutants that survive without iron-sulfur clusters. |
| ジャーナル | Mol Microbiol |
| Abstract |
Biological assembly of iron-sulfur (Fe-S) clusters is mediated by complex systems consisting of multiple proteins. Escherichia coli possesses two distinct systems called the ISC and SUF machineries encoded by iscSUA-hscBA-fdx-iscX and sufABCDSE respectively. Deletion of both pathways results in absence of the biosynthetic apparatus for Fe-S clusters, and consequent lethality, which has hampered detailed genetic studies. Here we report that modification of the isoprenoid biosynthetic pathway can offset the indispensability of the Fe-S cluster biosynthetic systems and show that the resulting Δisc Δsuf double mutants can grow without detectable Fe-S cluster-containing proteins. We also constructed a series of mutants in which each isc gene was disrupted in the deletion background of sufABCDSE. Phenotypic analysis of the mutants revealed that Fdx, an essential electron-transfer Fe-S protein in the ISC machinery, is dispensable under anaerobic conditions, which is similar to the situation with IscA. Furthermore, we found that several suppressor mutations in IscU, an Fe-S scaffold protein responsible for the de novo Fe-S cluster assembly, could bypass the essential role of the chaperone system HscA and HscB. These findings pave the way toward a detailed molecular analysis to understand the mechanisms involved in Fe-S cluster biosynthesis. |
| 巻・号 | 99(5) |
| ページ | 835-48 |
| 公開日 | 2016-3-1 |
| DOI | 10.1111/mmi.13271 |
| PMID | 26560204 |
| MeSH | Biosynthetic Pathways Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli Proteins / metabolism Genetic Association Studies Iron / metabolism Iron-Sulfur Proteins / genetics* Iron-Sulfur Proteins / metabolism* Multigene Family Mutation Sulfur / metabolism Terpenes / metabolism |
| IF | 3.418 |
| 引用数 | 24 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |