RRC ID 5374
著者 Hedi A, Fardeau ML, Sadfi N, Boudabous A, Ollivier B, Cayol JL.
タイトル Characterization of Halanaerobaculum tunisiense gen. nov., sp. nov., a new halophilic fermentative, strictly anaerobic bacterium isolated from a hypersaline lake in Tunisia.
ジャーナル Extremophiles
Abstract A new halophilic anaerobe was isolated from the hypersaline surface sediments of El-Djerid Chott, Tunisia. The isolate, designated as strain 6SANG, grew at NaCl concentrations ranging from 14 to 30%, with an optimum at 20-22%. Strain 6SANG was a non-spore-forming, non-motile, rod-shaped bacterium, appearing singly, in pairs, or occasionally as long chains (0.7-1 x 4-13 microm) and showed a Gram-negative-like cell wall pattern. It grew optimally at pH values between 7.2 and 7.4, but had a very broad pH range for growth (5.9-8.4). Optimum temperature for growth was 42 degrees C (range 30-50 degrees C). Strain 6SANG required yeast extract for growth on sugars. Glucose, sucrose, galactose, mannose, maltose, cellobiose, pyruvate, and starch were fermented. The end products from glucose fermentation were acetate, butyrate, lactate, H(2), and CO(2). The G + C ratio of the DNA was 34.3 mol%. Strain 6SANG exhibited 16S rRNA gene sequence similarity values of 91-92% with members of the genus Halobacteroides, H. halobius being its closest phylogenetic relative. Based on phenotypic and phylogenetic characteristics, we propose that this bacterium be classified as a novel species of a novel genus, Halanaerobaculum tunisiense gen. nov., sp. nov. The type strain is 6SANG(T) (=DSM 19997(T)=JCM 15060(T)).
巻・号 13(2)
ページ 313-9
公開日 2009-3-1
DOI 10.1007/s00792-008-0218-y
PMID 19115035
MeSH Bacteria, Anaerobic / classification Bacteria, Anaerobic / genetics* Bacteria, Anaerobic / metabolism Bacterial Typing Techniques DNA, Bacterial / chemistry Fatty Acids / chemistry Geologic Sediments / microbiology Hydrogen-Ion Concentration Microscopy, Electron / methods Microscopy, Phase-Contrast / methods Phylogeny RNA, Ribosomal, 16S / chemistry Seawater / microbiology Tunisia
IF 2.462
引用数 11
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM15060