| RRC ID |
53742
|
| 著者 |
Nomura Y, Ohno S, Nishikawa K, Yokogawa T.
|
| タイトル |
Correlation between the stability of tRNA tertiary structure and the catalytic efficiency of a tRNA-modifying enzyme, archaeal tRNA-guanine transglycosylase.
|
| ジャーナル |
Genes Cells
|
| Abstract |
In many archaeal tRNAs, archaeosine is found at position 15. During archaeosine biosynthesis, archaeal tRNA-guanine transglycosylase (ArcTGT) first replaces the guanine base at position 15 with 7-cyano-7-deazaguanine (preQ0). In this study, we investigated whether modified nucleosides in tRNA substrates would affect ArcTGT incorporation of preQ0. We prepared a series of hypomodified tRNAs(Ser)(GGA) from Escherichia coli strains lacking each tRNA-modifying enzyme. Measurement of ArcTGT kinetic parameters with the various tRNAs(Ser)(GGA) as substrates showed that the Km decreased due to the lack of modified nucleosides. The tRNAs(Ser)(GGA) melting profiles resulted in experimental evidence showing that each modified nucleoside in tRNA(Ser)(GGA) enhanced tRNA stability. Furthermore, the ArcTGT K(m) strongly correlated with the melting temperature (T(m)), suggesting that the unstable tRNA containing fewer modified nucleosides served as a better ArcTGT substrate. These results show that preQ0 incorporation into tRNA by ArcTGT takes place early in the archaeal tRNA modification process.
|
| 巻・号 |
21(1)
|
| ページ |
41-52
|
| 公開日 |
2016-1-1
|
| DOI |
10.1111/gtc.12317
|
| PMID |
26663416
|
| MeSH |
Archaea / enzymology
Archaea / genetics
Base Sequence
Biocatalysis*
Escherichia coli / metabolism
Guanine / metabolism
Kinetics
Methanosarcina / enzymology*
Molecular Sequence Data
Nucleic Acid Conformation*
Pentosyltransferases / metabolism*
RNA Stability / genetics*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Transfer / chemistry*
RNA, Transfer / genetics
RNA, Transfer / metabolism*
Substrate Specificity
Transition Temperature
|
| IF |
1.655
|
| 引用数 |
7
|
| リソース情報 |
| 原核生物(大腸菌) |
|
