RRC ID 53743
Author Schmidt A, Kochanowski K, Vedelaar S, Ahrné E, Volkmer B, Callipo L, Knoops K, Bauer M, Aebersold R, Heinemann M.
Title The quantitative and condition-dependent Escherichia coli proteome.
Journal Nat. Biotechnol.
Abstract Measuring precise concentrations of proteins can provide insights into biological processes. Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art quantitative mass spectrometry techniques to generate a comprehensive, condition-dependent protein-abundance map for Escherichia coli. We measure cellular protein concentrations for 55% of predicted E. coli genes (>2,300 proteins) under 22 different experimental conditions and identify methylation and N-terminal protein acetylations previously not known to be prevalent in bacteria. We uncover system-wide proteome allocation, expression regulation and post-translational adaptations. These data provide a valuable resource for the systems biology and broader E. coli research communities.
Volume 34(1)
Pages 104-10
Published 2016-1
DOI 10.1038/nbt.3418
PII nbt.3418
PMID 26641532
PMC PMC4888949
MeSH Escherichia coli / metabolism* Escherichia coli Proteins / metabolism* Mass Spectrometry Proteome*
IF 31.864
Resource
Prokaryotes E. coli