RRC ID 53759
Author Orman MA, Brynildsen MP.
Title Persister formation in Escherichia coli can be inhibited by treatment with nitric oxide.
Journal Free Radic. Biol. Med.
Abstract Bacterial persisters are phenotypic variants that survive extraordinary concentrations of antibiotics, and are thought to underlie the propensity of biofilm infections to relapse. Unfortunately many aspects of persister physiology remain ill-defined, which prevents progress toward eradicating the phenotype. Recently, we identified respiration within non-growing Escherichia coli populations as a potential target for the elimination type I persisters, which are those that arise from passage through stationary phase. Here we discovered that nitric oxide (NO) treatment at the onset of stationary phase significantly reduced type I persister formation through its ability to inhibit respiration. NO decreased protein and RNA degradation in stationary phase cells, and produced populations that were more fit for protein synthesis and growth resumption upon introduction into fresh media than untreated controls. Overall, this data shows that NO, which is a therapeutically-relevant compound, has the potential to decrease the incidence of recurrent infections from persisters.
Volume 93
Pages 145-54
Published 2016-4
DOI 10.1016/j.freeradbiomed.2016.02.003
PII S0891-5849(16)00046-0
PMID 26849946
PMC PMC4898466
MeSH Anti-Bacterial Agents / adverse effects Biofilms / drug effects* Biofilms / growth & development Drug Resistance, Bacterial / drug effects* Escherichia coli / drug effects Escherichia coli / growth & development Escherichia coli / metabolism* Humans Microbial Sensitivity Tests Nitric Oxide / metabolism Nitric Oxide / pharmacology* Protein Biosynthesis Proteolysis / drug effects RNA Stability
IF 5.657
Times Cited 13
Prokaryotes E. coli