論文 - 詳細
| RRC ID | 53766 |
|---|---|
| 著者 | Sakai Y, Miyauchi K, Kimura S, Suzuki T. |
| タイトル | Biogenesis and growth phase-dependent alteration of 5-methoxycarbonylmethoxyuridine in tRNA anticodons. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Post-transcriptional modifications at the anticodon first (wobble) position of tRNA play critical roles in precise decoding of genetic codes. 5-carboxymethoxyuridine (cmo(5)U) and its methyl ester derivative 5-methoxycarbonylmethoxyuridine (mcmo(5)U) are modified nucleosides found at the anticodon wobble position in several tRNAs from Gram-negative bacteria. cmo(5)U and mcmo(5)U facilitate non-Watson-Crick base pairing with guanosine and pyrimidines at the third positions of codons, thereby expanding decoding capabilities. By mass spectrometric analyses of individual tRNAs and a shotgun approach of total RNA from Escherichia coli, we identified mcmo(5)U as a major modification in tRNA(Ala1), tRNA(Ser1), tRNA(Pro3) and tRNA(Thr4); by contrast, cmo(5)U was present primarily in tRNA(Leu3) and tRNA(Val1). In addition, we discovered 5-methoxycarbonylmethoxy-2'-O-methyluridine (mcmo(5)Um) as a novel but minor modification in tRNA(Ser1). Terminal methylation frequency of mcmo(5)U in tRNA(Pro3) was low (≈30%) in the early log phase of cell growth, gradually increased as growth proceeded and reached nearly 100% in late log and stationary phases. We identified CmoM (previously known as SmtA), an AdoMet-dependent methyltransferase that methylates cmo(5)U to form mcmo(5)U. A luciferase reporter assay based on a +1 frameshift construct revealed that terminal methylation of mcmo(5)U contributes to the decoding ability of tRNA(Ala1). |
| 巻・号 | 44(2) |
| ページ | 509-23 |
| 公開日 | 2016-1-29 |
| DOI | 10.1093/nar/gkv1470 |
| PII | gkv1470 |
| PMID | 26681692 |
| PMC | PMC4737166 |
| MeSH | Amino Acid Sequence Anticodon / chemistry Anticodon / metabolism* Base Pairing Codon / chemistry Codon / metabolism* Escherichia coli / genetics* Escherichia coli / metabolism Gammaproteobacteria / genetics Gammaproteobacteria / metabolism Gene Expression Regulation, Bacterial Genetic Code Models, Molecular Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial / chemistry RNA, Bacterial / metabolism* RNA, Transfer / chemistry RNA, Transfer / metabolism* Ribosomes / genetics Ribosomes / metabolism Sequence Alignment Uridine / analogs & derivatives Uridine / metabolism* |
| IF | 11.502 |
| 引用数 | 16 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |