論文 - 詳細
| RRC ID | 53788 |
|---|---|
| 著者 | Xu Q, Shoji M, Shibata S, Naito M, Sato K, Elsliger MA, Grant JC, Axelrod HL, Chiu HJ, Farr CL, Jaroszewski L, Knuth MW, Deacon AM, Godzik A, Lesley SA, Curtis MA, Nakayama K, Wilson IA. |
| タイトル | A Distinct Type of Pilus from the Human Microbiome. |
| ジャーナル | Cell |
| Abstract |
Pili are proteinaceous polymers of linked pilins that protrude from the cell surface of many bacteria and often mediate adherence and virulence. We investigated a set of 20 Bacteroidia pilins from the human microbiome whose structures and mechanism of assembly were unknown. Crystal structures and biochemical data revealed a diverse protein superfamily with a common Greek-key β sandwich fold with two transthyretin-like repeats that polymerize into a pilus through a strand-exchange mechanism. The assembly mechanism of the central, structural pilins involves proteinase-assisted removal of their N-terminal β strand, creating an extended hydrophobic groove that binds the C-terminal donor strands of the incoming pilin. Accessory pilins at the tip and base have unique structural features specific to their location, allowing initiation or termination of the assembly. The Bacteroidia pilus, therefore, has a biogenesis mechanism that is distinct from other known pili and likely represents a different type of bacterial pilus. |
| 巻・号 | 165(3) |
| ページ | 690-703 |
| 公開日 | 2016-4-21 |
| DOI | 10.1016/j.cell.2016.03.016 |
| PII | S0092-8674(16)30272-0 |
| PMID | 27062925 |
| PMC | PMC4842110 |
| MeSH | Amino Acid Sequence Crystallography, X-Ray Fimbriae Proteins / chemistry* Fimbriae Proteins / genetics Fimbriae Proteins / metabolism Fimbriae, Bacterial* Gastrointestinal Microbiome* Humans Lipoproteins / chemistry Lipoproteins / metabolism Models, Molecular Molecular Sequence Data Sequence Alignment |
| IF | 38.637 |
| 引用数 | 24 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 38 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | S17-1 BL21(DE3) |