| RRC ID |
5379
|
| 著者 |
Takizawa T, Gudla PR, Guo L, Lockett S, Misteli T.
|
| タイトル |
Allele-specific nuclear positioning of the monoallelically expressed astrocyte marker GFAP.
|
| ジャーナル |
Genes Dev
|
| Abstract |
Chromosomes and genes are nonrandomly arranged within the mammalian cell nucleus. However, the functional significance of nuclear positioning in gene expression is unclear. Here we directly probed the relationship between nuclear positioning and gene activity by comparing the location of the active and inactive copies of a monoallelically expressed gene in single cell nuclei. We demonstrate that the astrocyte-specific marker GFAP (glial fibrillary acidic protein) is monoallelically expressed in cortical astrocytes. Selection of the active allele occurs in a stochastic manner and is generally maintained through cell division. Taking advantage of the monoallelic expression of GFAP, we show that the functionally distinct alleles occupy differential radial positions within the cell nucleus and differentially associate with intranuclear compartments. In addition, coordinately regulated astrocyte-specific genes on distinct chromosomes spatially associate in their inactive state and dissociate upon activation. These results provide direct evidence for function-related differential positioning of individual gene alleles within the interphase nucleus.
|
| 巻・号 |
22(4)
|
| ページ |
489-98
|
| 公開日 |
2008-2-15
|
| DOI |
10.1101/gad.1634608
|
| PII |
22/4/489
|
| PMID |
18281462
|
| PMC |
PMC2238670
|
| MeSH |
Alleles*
Animals
Astrocytes / physiology*
Biomarkers / metabolism*
Cell Differentiation*
Cell Nucleus / genetics*
Cell Nucleus / metabolism
Cell Nucleus / ultrastructure
Glial Fibrillary Acidic Protein / genetics
Glial Fibrillary Acidic Protein / physiology*
Immunoblotting
In Situ Hybridization, Fluorescence
Mice
RNA, Messenger / genetics
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
9.527
|
| 引用数 |
100
|
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00408 |
