RRC ID 53797
Author Nanamiya H, Ohashi Y, Asai K, Moriya S, Ogasawara N, Fujita M, Sadaie Y, Kawamura F.
Title ClpC regulates the fate of a sporulation initiation sigma factor, sigmaH protein, in Bacillus subtilis at elevated temperatures.
Journal Mol Microbiol
Abstract Using a strain carrying a clpC-bgaB transcriptional fusion at the amyE locus, we found that the expression of a clpC operon was induced at the end of exponential growth in a sigmaB-independent manner and ceased around T3.5 in the wild type but not in a spo0H mutant. This suggests that some gene product(s) whose expression is dependent on sigmaH function is required for the turn-off of clpC transcription during an early stage of sporulation. A clpC deletion mutant showed a temperature-sensitive sporulation phenotype and exhibited an abnormally large accumulation of sigmaH in the cell at 45 degrees C after T2, at which time the sigmaH level in the wild type had begun to decrease. These results, together with the fact that spo0H transcription in the clpC deletion mutant was similar to that of the wild type, suggested that ClpC may be responsible for the degradation of sigmaH after the accomplishment of its role in sporulation. Moreover, as expected from these results, overproduction of Spo0A was also observed after the initiation of sporulation in the clpC deletion mutant at 45 degrees C.
Volume 29(2)
Pages 505-13
Published 1998-7-1
DOI 10.1046/j.1365-2958.1998.00943.x
PMID 9720868
MeSH Bacillus subtilis / physiology* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Gene Deletion Gene Expression Regulation, Bacterial Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism* Hot Temperature Mutation Operon Spores, Bacterial / metabolism Transcription Factors / genetics Transcription Factors / metabolism*
IF 3.418
Times Cited 54
Prokaryotes B. subtilis MBS663 MBS664 MBS665