| RRC ID |
53797
|
| 著者 |
Nanamiya H, Ohashi Y, Asai K, Moriya S, Ogasawara N, Fujita M, Sadaie Y, Kawamura F.
|
| タイトル |
ClpC regulates the fate of a sporulation initiation sigma factor, sigmaH protein, in Bacillus subtilis at elevated temperatures.
|
| ジャーナル |
Mol Microbiol
|
| Abstract |
Using a strain carrying a clpC-bgaB transcriptional fusion at the amyE locus, we found that the expression of a clpC operon was induced at the end of exponential growth in a sigmaB-independent manner and ceased around T3.5 in the wild type but not in a spo0H mutant. This suggests that some gene product(s) whose expression is dependent on sigmaH function is required for the turn-off of clpC transcription during an early stage of sporulation. A clpC deletion mutant showed a temperature-sensitive sporulation phenotype and exhibited an abnormally large accumulation of sigmaH in the cell at 45 degrees C after T2, at which time the sigmaH level in the wild type had begun to decrease. These results, together with the fact that spo0H transcription in the clpC deletion mutant was similar to that of the wild type, suggested that ClpC may be responsible for the degradation of sigmaH after the accomplishment of its role in sporulation. Moreover, as expected from these results, overproduction of Spo0A was also observed after the initiation of sporulation in the clpC deletion mutant at 45 degrees C.
|
| 巻・号 |
29(2)
|
| ページ |
505-13
|
| 公開日 |
1998-7-1
|
| DOI |
10.1046/j.1365-2958.1998.00943.x
|
| PMID |
9720868
|
| MeSH |
Bacillus subtilis / physiology*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Gene Deletion
Gene Expression Regulation, Bacterial
Heat-Shock Proteins / genetics
Heat-Shock Proteins / metabolism*
Hot Temperature
Mutation
Operon
Spores, Bacterial / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism*
|
| IF |
3.418
|
| 引用数 |
54
|
| リソース情報 |
| 原核生物(枯草菌) |
MBS663
MBS664
MBS665 |
