RRC ID 53802
著者 Asai K, Inaoka T, Nanamiya H, Sadaie Y, Ochi K, Kawamura F.
タイトル Isolation and characterization of sporulation-initiation mutation in the Bacillus subtilis prfB gene.
ジャーナル Biosci Biotechnol Biochem
Abstract A Bacillus subtilis prfB45 mutant grew at 42 degrees C, but its sporulation was severely defective at 37 degrees C. Sporulation-specific induction of kinA, spo0A, and spo0H genes was inhibited in the mutant. The effects of temperature up-shift and down-shift on sporulation of the prfB45 mutant was observed at an early stage of sporulation. UGA readthrough frequency at non-permissive temperatures for sporulation was higher in the mutant than in the wild-type strain. Temperature-sensitive sporulation of the prfB45 mutant was suppressed by mutations in rpsL coding for S12 of ribosomes, required for accurate termination of translation. Additionally, spontaneous second-site mutations that suppressed the sporulation phenotype of the prfB45 strain were found in the rpoB gene. These results suggest that accurate termination of translation is required for proper initiation of sporulation.
巻・号 71(2)
ページ 397-406
公開日 2007-2-1
DOI 10.1271/bbb.60445
PII JST.JSTAGE/bbb/60445
PMID 17284856
MeSH Bacillus subtilis / genetics* Bacillus subtilis / growth & development Bacillus subtilis / isolation & purification Codon, Nonsense / genetics Culture Media Genes, Bacterial / genetics* Genes, Suppressor Mutation / physiology Phenotype Spores, Bacterial / genetics* Temperature beta-Galactosidase / chemistry beta-Galactosidase / genetics beta-Galactosidase / metabolism
IF 1.516
引用数 7
リソース情報
原核生物(枯草菌) MBS662