論文 - 詳細
| RRC ID | 53812 |
|---|---|
| 著者 | Mitani T, Watanabe S, Yoshioka Y, Katayama S, Nakamura S, Ashida H. |
| タイトル | Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein β degradation by adenosine receptor A1. |
| ジャーナル | Biochim Biophys Acta Mol Cell Res |
| Abstract |
Theobromine, a methylxanthine derived from cacao beans, reportedly has various health-promoting properties but molecular mechanism by which effects of theobromine on adipocyte differentiation and adipogenesis remains unclear. In this study, we aimed to clarify the molecular mechanisms of the anti-adipogenic effect of theobromine in vitro and in vivo. ICR mice (4week-old) were administered with theobromine (0.1g/kg) for 7days. Theobromine administration attenuated gains in body and epididymal adipose tissue weights in mice and suppressed expression of adipogenic-associated genes in mouse adipose tissue. In 3T3-L1 preadipocytes, theobromine caused degradation of C/EBPβ protein by the ubiquitin-proteasome pathway. Pull down assay showed that theobromine selectively interacts with adenosine receptor A1 (AR1), and AR1 knockdown inhibited theobromine-induced C/EBPβ degradation. Theobromine increased sumoylation of C/EBPβ at Lys133. Expression of the small ubiquitin-like modifier (SUMO)-specific protease 2 (SENP2) gene, coding for a desumoylation enzyme, was suppressed by theobromine. In vivo knockdown studies showed that AR1 knockdown in mice attenuated the anti-adipogenic effects of theobromine in younger mice. Theobromine suppresses adipocyte differentiation and induced C/EBPβ degradation by increasing its sumoylation. Furthermore, the inhibition of AR1 signaling is important for theobromine-induced C/EBPβ degradation. |
| 巻・号 | 1864(12) |
| ページ | 2438-2448 |
| 公開日 | 2017-12-1 |
| DOI | 10.1016/j.bbamcr.2017.09.017 |
| PII | S0167-4889(17)30264-1 |
| PMID | 28965824 |
| MeSH | 3T3-L1 Cells Adipocytes / metabolism Adipogenesis / drug effects Adipogenesis / genetics* Animals CCAAT-Enhancer-Binding Protein-beta / metabolism* Cell Differentiation / genetics Cysteine Endopeptidases / genetics* Cysteine Endopeptidases / metabolism Mice Proteolysis / drug effects Receptors, Purinergic P1 / genetics* Signal Transduction Sumoylation / genetics Theobromine / administration & dosage |
| IF | 4.739 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | p3xFLAG-C/EBP beta (WT) (RDB15962) p3xFLAG-C/EBP beta (K133R) (RDB15963) |