RRC ID 53815
著者 Numata K, Horii Y, Oikawa K, Miyagi Y, Demura T, Ohtani M.
タイトル Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus.
ジャーナル Sci Rep
Abstract Cell-penetrating peptides (CPPs) are used for various applications, especially in the biomedical field. Recently, CPPs have been used as a part of carrier to deliver proteins and/or genes into plant cells and tissues; hence, these peptides are attractive tools for plant biotechnological and agricultural applications, but require more efficient delivery rates and optimization by species before wide-scale use can be achieved. Here, we developed a library containing 55 CPPs to determine the optimal CPP characteristics for penetration of BY-2 cells and leaves of Nicotiana benthamiana, Arabidopsis thaliana, tomato (Solanum lycopersicum), poplar (hybrid aspen Populus tremula × tremuloides line T89), and rice (Oryza sativa). By investigating the cell penetration efficiency of CPPs in the library, we identified several efficient CPPs for all the plants studied except rice leaf. In the case of rice, several CPPs showed efficient penetration into rice callus. Furthermore, we examined the relationship between cell penetration efficiency and CPP secondary structural characteristics. The cell penetration efficiency of Lys-containing CPPs was relatively greater in plant than in animal cells, which could be due to differences in lipid composition and surface charge of the cell membranes. The variation in optimal CPPs across the plants studied here suggests that CPPs must be optimized for each plant species and target tissues of interest.
巻・号 8(1)
ページ 10966
公開日 2018-7-20
DOI 10.1038/s41598-018-29298-6
PII 10.1038/s41598-018-29298-6
PMID 30030484
PMC PMC6054692
MeSH Animals Arabidopsis / chemistry Cell Line Cell Membrane / metabolism Cell-Penetrating Peptides / analysis* Gene Transfer Techniques* Oryza / chemistry Peptide Library* Plant Leaves / chemistry* Plant Leaves / metabolism Populus / chemistry Solanum lycopersicum / chemistry Tobacco / chemistry
IF 3.998
引用数 8
リソース情報
トマト TOMJPF00002(Moneymaker)
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00001