RRC ID 53827
Author Fujii M, Mizutani Y, Sakuma T, Tagami K, Okamoto K, Kuno Y, Harada M, Kubouchi K, Tsutsumi Y.
Title Corynebacterium kroppenstedtii in granulomatous mastitis: Analysis of formalin-fixed, paraffin-embedded biopsy specimens by immunostaining using low-specificity bacterial antisera and real-time polymerase chain reaction.
Journal Pathol Int
Abstract Granulomatous mastitis (GM) is a rare inflammatory disease of the post-lactation breast, clinically mimicking breast cancer. GM is microscopically characterized by formation of epithelioid granulomas and abscess (suppurative granulomas) with lipid droplet-centered inflammation. Corynebacterium kroppenstedtii (Ck) is known as a causative bacterium of GM, and identification of Ck infection within the lesion should thus be essential for confirming the diagnosis. In the present study, we analyzed formalin-fixed, paraffin-embedded (FFPE) biopsy specimens of a total of 18 GM lesions with immunostaining and real-time PCR for Ck genome. Widely cross-reactive rabbit antisera against Bacillus Calmette-Guerin (BCG), Bacillus cereus, Treponema pallidum and Escherichia coli were chosen. With real-time PCR, Ck genome was demonstrated in 7 of 18 GM lesions. Immunohistochemically, the low-specificity antisera reacted with the cytoplasm of phagocytes and/or granuloma-engulfed lipid droplets in 12 of 18 GM lesions. Antigenic positivity was observed in the following order: BCG > B. cereus > T. pallidum > E. coli. Real-time PCR using DNA extracted from FFPE sections was useful but not consistent for identifying the Ck genome in GM, while immunostaining using cross-reactive antisera against four kinds of bacteria was not Ck-specific but was applicable to visualizing bacterial infection within the GM lesions.
Volume 68(7)
Pages 409-418
Published 2018-7-1
DOI 10.1111/pin.12683
PMID 29862601
MeSH Adult Antibodies, Bacterial / immunology Antibody Specificity Corynebacterium / immunology Corynebacterium Infections / diagnosis* Cross Reactions Female Formaldehyde Granulomatous Mastitis / diagnosis* Granulomatous Mastitis / microbiology* Humans Immunohistochemistry / methods Middle Aged Paraffin Embedding Real-Time Polymerase Chain Reaction / methods Tissue Fixation
IF 2.11
Times Cited 4
DNA material Genomic DNA of Corynebacterium kroppenstedtii JCM 11950 T (JGD07466) Genomic DNA of Propionibacterium acnes JCM 6425 T (JGD07462)