| RRC ID |
53827
|
| Author |
Fujii M, Mizutani Y, Sakuma T, Tagami K, Okamoto K, Kuno Y, Harada M, Kubouchi K, Tsutsumi Y.
|
| Title |
Corynebacterium kroppenstedtii in granulomatous mastitis: Analysis of formalin-fixed, paraffin-embedded biopsy specimens by immunostaining using low-specificity bacterial antisera and real-time polymerase chain reaction.
|
| Journal |
Pathol Int
|
| Abstract |
Granulomatous mastitis (GM) is a rare inflammatory disease of the post-lactation breast, clinically mimicking breast cancer. GM is microscopically characterized by formation of epithelioid granulomas and abscess (suppurative granulomas) with lipid droplet-centered inflammation. Corynebacterium kroppenstedtii (Ck) is known as a causative bacterium of GM, and identification of Ck infection within the lesion should thus be essential for confirming the diagnosis. In the present study, we analyzed formalin-fixed, paraffin-embedded (FFPE) biopsy specimens of a total of 18 GM lesions with immunostaining and real-time PCR for Ck genome. Widely cross-reactive rabbit antisera against Bacillus Calmette-Guerin (BCG), Bacillus cereus, Treponema pallidum and Escherichia coli were chosen. With real-time PCR, Ck genome was demonstrated in 7 of 18 GM lesions. Immunohistochemically, the low-specificity antisera reacted with the cytoplasm of phagocytes and/or granuloma-engulfed lipid droplets in 12 of 18 GM lesions. Antigenic positivity was observed in the following order: BCG > B. cereus > T. pallidum > E. coli. Real-time PCR using DNA extracted from FFPE sections was useful but not consistent for identifying the Ck genome in GM, while immunostaining using cross-reactive antisera against four kinds of bacteria was not Ck-specific but was applicable to visualizing bacterial infection within the GM lesions.
|
| Volume |
68(7)
|
| Pages |
409-418
|
| Published |
2018-7-1
|
| DOI |
10.1111/pin.12683
|
| PMID |
29862601
|
| MeSH |
Adult
Antibodies, Bacterial / immunology
Antibody Specificity
Corynebacterium / immunology
Corynebacterium Infections / diagnosis*
Cross Reactions
Female
Formaldehyde
Granulomatous Mastitis / diagnosis*
Granulomatous Mastitis / microbiology*
Humans
Immunohistochemistry / methods
Middle Aged
Paraffin Embedding
Real-Time Polymerase Chain Reaction / methods
Tissue Fixation
|
| IF |
2.11
|
| Times Cited |
4
|
| Resource |
| DNA material |
Genomic DNA of Corynebacterium kroppenstedtii JCM 11950 T (JGD07466)
Genomic DNA of Propionibacterium acnes JCM 6425 T (JGD07462) |
