| RRC ID |
53862
|
| 著者 |
Abdelmoez MN, Iida K, Oguchi Y, Nishikii H, Yokokawa R, Kotera H, Uemura S, Santiago JG, Shintaku H.
|
| タイトル |
SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology.
|
| ジャーナル |
Genome Biol
|
| Abstract |
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gene expression in the subcellular compartments, and combines them to create novel single-cell RNA-seq data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA and nucRNA that reflect the transient physiological state of single cells. These data provide unique insights into the regulatory network of messenger RNA from the nucleus toward the cytoplasm at the single-cell level.
|
| 巻・号 |
19(1)
|
| ページ |
66
|
| 公開日 |
2018-6-6
|
| DOI |
10.1186/s13059-018-1446-9
|
| PII |
10.1186/s13059-018-1446-9
|
| PMID |
29871653
|
| PMC |
PMC5989370
|
| MeSH |
Cell Line, Tumor
Cell Nucleus / genetics*
Cell Physiological Phenomena / genetics*
Cytoplasm / genetics*
Gene Expression / genetics*
Gene Library
High-Throughput Nucleotide Sequencing / methods
Humans
K562 Cells
RNA / genetics
Sequence Analysis, RNA / methods
Single-Cell Analysis / methods
|
| IF |
10.806
|
| 引用数 |
10
|
| リソース情報 |
| ヒト・動物細胞 |
K562(RCB0027) |
