RRC ID |
53935
|
著者 |
Kinashi T, Harada N, Severinson E, Tanabe T, Sideras P, Konishi M, Azuma C, Tominaga A, Bergstedt-Lindqvist S, Takahashi M.
|
タイトル |
Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II.
|
ジャーナル |
Nature
|
Abstract |
Proliferation and maturation of antigen-stimulated B cells are regulated by several soluble factors derived from macrophages and T cells. These soluble factors are functionally divided into two groups: B-cell growth factor (BCGF), thought to be involved in B-cell proliferation; and B-cell differentiation factor (BCDF), responsible for maturation of activated B cells into immunoglobulin-secreting cells. This classification needs to be re-examined in the light of the recent cloning of complementary DNA encoding IgG1 induction factor (interleukin-4, IL-4) from the 2.19 mouse T-cell line. Recombinant IL-4 has BCGF and BCDF activities and affects B cells, T cells and mast cells (refs 7, 8; our unpublished data). Another well-characterized B-cell factor is T-cell replacing factor (TRF), which, when secreted by the murine T-cell hybridoma B151K12, is defined by two activities: induction of IgM secretion by BCL1 leukaemic B-cell line; and induction of secondary anti-dinitrophenol (DNP) immunoglobulin G (IgG) synthesis in vitro by DNP-prime B cells. Although TRF from B151K12 was classified as BCDF, purified TRF has BCGF-II activity. To elucidate the molecular properties of TRF we isolated cDNA encoding TRF from the 2.19 T-cell line and report here the structure and multiple activities of this lymphokine.
|
巻・号 |
324(6092)
|
ページ |
70-3
|
公開日 |
1986-11-6
|
DOI |
10.1038/324070a0
|
PMID |
3024009
|
MeSH |
Animals
Base Sequence
Cloning, Molecular
DNA / metabolism
DNA Restriction Enzymes / metabolism
Growth Substances / metabolism*
Interleukin-4
Interleukin-5
Lymphokines / genetics
Lymphokines / metabolism*
Mice
|
IF |
42.779
|
引用数 |
440
|
リソース情報 |
遺伝子材料 |
pSP6K. m . TRF23 (RDB01174) |