RRC ID 53998
著者 Ishigami K, Nosho K, Koide H, Kanno S, Mitsuhashi K, Igarashi H, Shitani M, Motoya M, Kimura Y, Hasegawa T, Kaneto H, Takemasa I, Suzuki H, Nakase H.
タイトル MicroRNA-31 reflects IL-6 expression in cancer tissue and is related with poor prognosis in bile duct cancer.
ジャーナル Carcinogenesis
Abstract Bile duct cancer is a highly aggressive malignancy wherein early diagnosis is difficult and few treatment options are available. MicroRNA-31 (miR-31) is reported to be related with survival in patients with gastrointestinal cancers; however, the regulatory mechanism of miR-31 and association between miR-31 expression and survival in patients with bile duct cancer cases have not been established. Thus, we evaluated miR-31 expression in bile duct cancer tissues and assessed its relationship with prognosis. Additionally, we examined the effects of several cytokines on miR-31 expression. The study included 81 samples of bile duct cancer tissues. MiR-31 expression in bile duct cancer cells was significantly higher than that in normal bile duct epithelial cells (P = 0.038). There were no significant associations between miR-31 expression and clinical or pathological characteristics, except for tumour size (P = 0.012). In Kaplan-Meier analysis, high miR-31 expression was significantly associated with shorter survival (log-rank test, P = 0.0082). In multivariate Cox regression analysis, high miR-31 expression was significantly associated with prognosis (P = 0.043), independent of clinical or pathological features. Interleukin-6 (IL-6) significantly promoted miR-31 expression and cell proliferation in a dose-dependent manner, and the inhibition of STAT-3 signalling significantly suppressed miR-31 expression and cell proliferation. In conclusion, high expression was significantly associated with poor prognosis in bile duct cancer patients. The IL-6-STAT-3 signalling regulated bile duct cancer cell proliferation and miR-31 expression. Our findings suggest that miR-31 may be a promising biomarker that reflects IL-6 expression in bile duct cancer tissues and predicts poor prognosis.
巻・号 39(9)
ページ 1127-1134
公開日 2018-9-21
DOI 10.1093/carcin/bgy075
PII 5025674
PMID 29860474
MeSH Aged Bile Duct Neoplasms / genetics* Bile Duct Neoplasms / mortality Bile Duct Neoplasms / pathology Cell Line, Tumor Cell Proliferation / genetics Cholangiocarcinoma / genetics* Cholangiocarcinoma / mortality Cholangiocarcinoma / pathology Female Gallbladder Neoplasms / genetics* Gallbladder Neoplasms / mortality Gallbladder Neoplasms / pathology Humans Interleukin-6 / biosynthesis* Male MicroRNAs / genetics* Prognosis STAT3 Transcription Factor / antagonists & inhibitors
IF 4.603
引用数 3
リソース情報
ヒト・動物細胞 TFK-1(RCB2537)