RRC ID |
53998
|
著者 |
Ishigami K, Nosho K, Koide H, Kanno S, Mitsuhashi K, Igarashi H, Shitani M, Motoya M, Kimura Y, Hasegawa T, Kaneto H, Takemasa I, Suzuki H, Nakase H.
|
タイトル |
MicroRNA-31 reflects IL-6 expression in cancer tissue and is related with poor prognosis in bile duct cancer.
|
ジャーナル |
Carcinogenesis
|
Abstract |
Bile duct cancer is a highly aggressive malignancy wherein early diagnosis is difficult and few treatment options are available. MicroRNA-31 (miR-31) is reported to be related with survival in patients with gastrointestinal cancers; however, the regulatory mechanism of miR-31 and association between miR-31 expression and survival in patients with bile duct cancer cases have not been established. Thus, we evaluated miR-31 expression in bile duct cancer tissues and assessed its relationship with prognosis. Additionally, we examined the effects of several cytokines on miR-31 expression. The study included 81 samples of bile duct cancer tissues. MiR-31 expression in bile duct cancer cells was significantly higher than that in normal bile duct epithelial cells (P = 0.038). There were no significant associations between miR-31 expression and clinical or pathological characteristics, except for tumour size (P = 0.012). In Kaplan-Meier analysis, high miR-31 expression was significantly associated with shorter survival (log-rank test, P = 0.0082). In multivariate Cox regression analysis, high miR-31 expression was significantly associated with prognosis (P = 0.043), independent of clinical or pathological features. Interleukin-6 (IL-6) significantly promoted miR-31 expression and cell proliferation in a dose-dependent manner, and the inhibition of STAT-3 signalling significantly suppressed miR-31 expression and cell proliferation. In conclusion, high expression was significantly associated with poor prognosis in bile duct cancer patients. The IL-6-STAT-3 signalling regulated bile duct cancer cell proliferation and miR-31 expression. Our findings suggest that miR-31 may be a promising biomarker that reflects IL-6 expression in bile duct cancer tissues and predicts poor prognosis.
|
巻・号 |
39(9)
|
ページ |
1127-1134
|
公開日 |
2018-9-21
|
DOI |
10.1093/carcin/bgy075
|
PII |
5025674
|
PMID |
29860474
|
MeSH |
Aged
Bile Duct Neoplasms / genetics*
Bile Duct Neoplasms / mortality
Bile Duct Neoplasms / pathology
Cell Line, Tumor
Cell Proliferation / genetics
Cholangiocarcinoma / genetics*
Cholangiocarcinoma / mortality
Cholangiocarcinoma / pathology
Female
Gallbladder Neoplasms / genetics*
Gallbladder Neoplasms / mortality
Gallbladder Neoplasms / pathology
Humans
Interleukin-6 / biosynthesis*
Male
MicroRNAs / genetics*
Prognosis
STAT3 Transcription Factor / antagonists & inhibitors
|
IF |
4.603
|
引用数 |
3
|
リソース情報 |
ヒト・動物細胞 |
TFK-1(RCB2537) |