RRC ID |
54025
|
著者 |
Sakoda M, Kaneko M, Ohta S, Qi P, Ichimura S, Yatomi Y, Ito T.
|
タイトル |
Injectable Hemostat Composed of a Polyphosphate-Conjugated Hyaluronan Hydrogel.
|
ジャーナル |
Biomacromolecules
|
Abstract |
We have developed a new hydrogel hemostat composed of hyaluronan (HA) conjugated with inorganic polyphosphate (PolyP). A hemostatic hydrogel, HAX-PolyP, was formed rapidly by mixing aldehyde-modified HA and hydrazide-modified HA conjugated with PolyP (HA-PolyP). Although the gelation rate decreased with increasing PolyP content, the gelation time was below 5 min. In addition, the hydrogel swelling volume decreased with increasing PolyP content, but the degradation rate did not depend on PolyP content and the hydrogel underwent complete degradation through hydrolysis over 3 weeks in phosphate buffered saline. HAX-PolyP showed similar biocompatibility with the HA hydrogel without PolyP conjugation in vitro and in vivo. Intraperitoneal administration of HAX-PolyP did not induce any adhesion in the peritoneum and clot formation in the lungs. Finally, HA-PolyP accelerated the coagulation rate of human plasma ex vivo, and HAX-PolyP showed as strong a hemostatic effect as fibrin glue in a mouse liver bleeding model in vivo.
|
巻・号 |
19(8)
|
ページ |
3280-3290
|
公開日 |
2018-8-13
|
DOI |
10.1021/acs.biomac.8b00588
|
PMID |
29985587
|
MeSH |
3T3 Cells
Animals
Hemostatics / administration & dosage
Hemostatics / chemistry*
Humans
Hyaluronic Acid / chemistry*
Hydrogels / chemistry*
Injections
Male
Mice
Mice, Inbred ICR
Polyphosphates / chemistry*
RAW 264.7 Cells
|
IF |
6.092
|
引用数 |
5
|
リソース情報 |
ヒト・動物細胞 |
RAW 264(RCB0535) |