RRC ID 54048
Author Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S.
Title Zfat expression in ZsGreen reporter gene knock‑in mice: Implications for a novel function of Zfat in definitive erythropoiesis.
Journal Int. J. Mol. Med.
Abstract Zinc finger and AT‑hook domain containing (Zfat) is a transcriptional regulator harboring an AT‑hook domain and 18 repeats of a C2H2 zinc‑finger motif, which binds directly to the proximal region of transcription start sites in Zfat‑target genes. It was previously reported that deletion of the Zfat gene in mice yields embryonic lethality by embryonic day 8.5 and impairs primitive hematopoiesis in yolk sac blood islands. In addition, Zfat has been reported to be involved in thymic T‑cell development and peripheral T‑cell homeostasis. In the present study, in order to obtain a precise understanding of the expression and function of Zfat, a knock‑in mouse strain (ZfatZsG/+ mice), which expressed ZsGreen in the Zfat locus, was established. ZsGreen signals in tissues and cells of ZfatZsG/+ mice were examined by flow cytometric and histological analyses. Consistent with our previous studies, ZsGreen signals in ZfatZsG/+ mice were detected in the embryo and yolk sac blood islands, as well as in thymocytes, B and T cells. In the ZfatZsG/+ thymus, ZsGreen+ cells were identified not only in T‑cell populations but also in thymic epithelial cells, suggesting the role of Zfat in antigen‑presenting cells during thymic T‑cell development. ZsGreen signals were observed in definitive erythroid progenitor cells in the fetal liver and adult bone marrow of ZfatZsG/+ mice. The proportion of ZsGreen+ cells in these tissues was highest at the early stage of erythroid differentiation, suggesting that Zfat serves particular roles in definitive erythropoiesis. Histological studies demonstrated that ZsGreen signals were detected in the pyramidal cells in the hippocampal CA1 region and the Purkinje cells in the cerebellum, suggesting novel functions of Zfat in nervous tissues. Taken together, these results indicated that the ZfatZsG/+ reporter mouse may be considered a useful tool for elucidating the expression and function of Zfat.
Volume 42(5)
Pages 2595-2603
Published 2018-11
DOI 10.3892/ijmm.2018.3806
PMID 30106088
PMC PMC6192767
MeSH Animals B-Lymphocytes / cytology B-Lymphocytes / metabolism CA1 Region, Hippocampal / cytology CA1 Region, Hippocampal / metabolism Cell Differentiation / physiology Erythropoiesis / genetics Erythropoiesis / physiology* Gene Expression Regulation / physiology Gene Knock-In Techniques Mice Mice, Mutant Strains Purkinje Cells / cytology Purkinje Cells / metabolism T-Lymphocytes / cytology T-Lymphocytes / metabolism Thymocytes / cytology Thymocytes / metabolism Transcription Factors / genetics Transcription Factors / metabolism*
IF 2.784
Resource
Human and Animal Cells TT2(AES0014)