論文 - 詳細
| RRC ID | 54063 |
|---|---|
| 著者 | Png E, Hou A, Tong L. |
| タイトル | Mechanistic role of transglutaminase-2 in focal adhesions. |
| ジャーナル | Sci Rep |
| Abstract |
Transglutaminase (TG)-2 interacts with matrix proteins and integrins, forming focal adhesions (FA) to initiate cell migration, thus playing a vital role in wound healing. Previously we showed that TG-2 influenced phosphorylation of paxillin and other FA proteins. Here, we aimed to investigate the molecular mechanism of TG-2 regulation of paxillin. Human corneal epithelial cells expressing shRNA against TG-2 (shTG) and scrambled sequence control (shRNA) were cultured. TG-2 was pulled down by anti-paxillin antibody, but not MAP3K12. Cell-free interaction assay with immobilized paxillin shows that TG-2 bind to paxillin directly. JNK was the strongest kinase for paxillin phosphorylation in the in-vitro kinase screen, but TG-2 could not phosphorylate paxillin directly. Increasing TG-2 concentrations did not increase the amount of JNK in the TG-2/paxillin complex. Immunofluoresent staining shows that TG-2 colocalises with vinculin and paxillin in FA of migrating cells. TG-2 binds to paxillin and JNK-containing FA but does not recruit JNK directly. Taken together with previous findings, TG-2 binds paxillin non-covalently, and JNK can phosphorylate paxillin, these processes critically regulate corneal epithelial adhesion and migration. |
| 巻・号 | 8(1) |
| ページ | 12370 |
| 公開日 | 2018-8-17 |
| DOI | 10.1038/s41598-018-30172-8 |
| PII | 10.1038/s41598-018-30172-8 |
| PMID | 30120307 |
| PMC | PMC6098073 |
| MeSH | Blotting, Western Cell Line Cell Movement / genetics Cell Movement / physiology Focal Adhesions / genetics Focal Adhesions / metabolism* GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism* Humans Immunoprecipitation MAP Kinase Signaling System / genetics MAP Kinase Signaling System / physiology Mass Spectrometry Paxillin / genetics Paxillin / metabolism Phosphorylation / genetics Phosphorylation / physiology Protein Binding Protein Glutamine gamma Glutamyltransferase 2 RNA, Small Interfering Transglutaminases / genetics Transglutaminases / metabolism* |
| IF | 3.998 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |