論文 - 詳細
| RRC ID | 54089 |
|---|---|
| 著者 | Odkhuu E, Komatsu T, Koide N, Naiki Y, Takeuchi K, Tanaka Y, Tsolmongyn B, Jambalganiin U, Morita N, Yoshida T, Gotoh B, Yokochi T. |
| タイトル | Sendai virus C protein limits NO production in infected RAW264.7 macrophages. |
| ジャーナル | Innate Immun |
| Abstract |
To suppress virus multiplication, infected macrophages produce NO. However, it remains unclear how infecting viruses then overcome NO challenge. In the present study, we report the effects of accessory protein C from Sendai virus (SeV), a prototypical paramyxovirus, on NO output. We found that in RAW264.7 murine macrophages, a mutant SeV without C protein (4C(-)) significantly enhanced inducible NO synthase (iNOS) expression and subsequent NO production compared to wild type SeV (wtSeV). SeV 4C(-) infection caused marked production of IFN-β, which is involved in induction of iNOS expression via the JAK-STAT pathway. Addition of anti-IFN-β Ab, however, resulted in only marginal suppression of NO production. In contrast, NF-κB, a primarily important factor for transcription of the iNOS gene, was also activated by 4C(-) infection but not wtSeV infection. Induction of NO production and iNOS expression by 4C(-) was significantly suppressed in cells constitutively expressing influenza virus NS1 protein that can sequester double-stranded (ds)RNA, which triggers activation of signaling pathways leading to activation of NF-κB and IRF3. Therefore, C protein appears to suppress NF-κB activation to inhibit iNOS expression and subsequent NO production, possibly by limiting dsRNA generation in the context of viral infection. |
| 巻・号 | 24(7) |
| ページ | 430-438 |
| 公開日 | 2018-10-1 |
| DOI | 10.1177/1753425918796619 |
| PMID | 30189760 |
| PMC | PMC6830875 |
| MeSH | Animals Gene Expression Regulation Interferon Regulatory Factor-3 / metabolism Janus Kinases / metabolism Macrophages / physiology* Mice Mutation / genetics NF-kappa B / metabolism Nitric Oxide / metabolism Nitric Oxide Synthase Type II / genetics Nitric Oxide Synthase Type II / metabolism RAW 264.7 Cells RNA, Double-Stranded / metabolism Respirovirus Infections / immunology* STAT Transcription Factors / metabolism Sendai virus / physiology* Signal Transduction Viral Nonstructural Proteins / metabolism Viral Proteins / genetics Viral Proteins / metabolism* |
| IF | 2.298 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |