論文 - 詳細
| RRC ID | 54102 |
|---|---|
| 著者 | Khalid H, Suhaib F, Zahid S, Ahmed S, Jamal A, Kaleem M, Khan AS. |
| タイトル | Microwave-assisted synthesis and in vitro osteogenic analysis of novel bioactive glass fibers for biomedical and dental applications. |
| ジャーナル | Biomed Mater |
| Abstract |
Glass fiber-based materials have gained interest for use in biomedical and dental applications. The aim of this study was to make E-glass fiber bioactive by a novel method using the microwave irradiation technique. Industrial E-glass fibers were used after surface activation with the hydrolysis method. The ratio of calcium and phosphorous precursors was set at 1.67. After maintaining the pH of the calcium solution, E-glass fibers in two ratios, i.e. 30% (nHA/E30) and 50% (nHA/E50) wt/wt, were added. The phosphorous precursor was added later and the solution was irradiated in a microwave to obtain nano-hydroxyapatite (nHA) particles on E-glass fibers. The structural, physical and in vitro biocompatibility analyses of the resulting materials were conducted. The expression of osteopontin (OPN) and collagen (Col) type 1 was measured by reverse transcription polymerase chain reaction (RT-PCR) and comparison was made between all the groups. Fourier transform infrared spectroscopy and x-ray diffraction showed characteristic peaks of nHA, and a change in the peak intensities was observed with an increase in the concentration of E-glass fibers. Scanning electron microscopic (SEM) images confirmed the homogenous adhesion of nHA spherical particles all over the fibers. Cell viability with mesenchymal stem cells showed growth, proliferation, and adhesion. All the materials were able to upregulate the expression of the OPN and Col, where gene expression was highest in nHA followed by nHA/E30 and nHA/E50. The bioactive glass fibers were synthesized in the shortest time and showed osteogenic properties. These materials have the potential for use in bone tissue engineering, dental prosthesis, and tooth restoration. |
| 巻・号 | 14(1) |
| ページ | 015005 |
| 公開日 | 2018-10-31 |
| DOI | 10.1088/1748-605X/aae3f0 |
| PMID | 30251708 |
| MeSH | 3T3 Cells Animals Biocompatible Materials / chemistry* Bone and Bones / metabolism Cell Adhesion Cell Enlargement Cell Proliferation Cell Survival Cells, Cultured Ceramics / chemistry* Collagen / chemistry DNA Primers / genetics Dental Materials / chemistry* Dental Prosthesis Dental Restoration Repair Femur / metabolism Mesenchymal Stem Cells / cytology Mice Microwaves* Osteoblasts / metabolism Osteogenesis Osteopontin / chemistry RNA / analysis Rats Tissue Engineering / methods |
| IF | 3.174 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |