Reference - Detail
| RRC ID | 54113 |
|---|---|
| Author | Kim M, Pyo S, Kang CH, Lee CO, Lee HK, Choi SU, Park CH. |
| Title | Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer. |
| Journal | PLoS One |
| Abstract |
Gastric cancer is a malignancy that has a high mortality rate. Although progress has been made in the treatment of gastric cancer, many patients experience cancer recurrence and metastasis. Folate receptor 1 (FOLR1) is overexpressed on the cell surface in over one-third of gastric cancer patients, but rarely is expressed in normal tissue. This makes FOLR1 a potential target for chimeric antigen receptor (CAR) T cell immunotherapy, although the function of FOLR1 has not been elucidated. CAR are engineered fusion receptor composed of an antigen recognition region and signaling domains. T cells expressing CAR have specific activation and cytotoxic effects against cancer cells containing the target antigen. In this study, we generated a CAR that targets FOLR1 composed of a single-chain variable fragment (scFv) of FOLR1 antibody and signaling domains consisting of CD28 and CD3ζ. Both FOLR1-CAR KHYG-1, a natural killer cell line, and FOLR1-CAR T cells recognized FOLR1-positive gastric cancer cells in a MHC-independent manner and induced secretion of various cytokines and caused cell death. Conclusively, this is the first study to demonstrate that CAR KHYG-1/T cells targeting FOLR1 are effective against FOLR1-positive gastric cancer cells. |
| Volume | 13(6) |
| Pages | e0198347 |
| Published | 2018-6-6 |
| DOI | 10.1371/journal.pone.0198347 |
| PII | PONE-D-18-05664 |
| PMID | 29874279 |
| PMC | PMC5991383 |
| MeSH | Animals Cell Line, Tumor Cell Proliferation Cell Survival Folate Receptor 1 / immunology* Humans Immunotherapy, Adoptive / methods* Jurkat Cells K562 Cells Mice Receptors, Antigen, T-Cell / immunology Receptors, Chimeric Antigen / immunology* Recombinant Fusion Proteins / immunology Stomach Neoplasms / immunology Stomach Neoplasms / therapy* T-Lymphocytes / immunology T-Lymphocytes / transplantation* Xenograft Model Antitumor Assays |
| IF | 2.74 |
| Times Cited | 14 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 26 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MKN7(RCB0999) GCIY(RCB0555) |