論文 - 詳細
| RRC ID | 54127 |
|---|---|
| 著者 | Mogi K, Hayashida K, Yamamoto T. |
| タイトル | Damage-less Handling of Exosomes Using an Ion-depletion Zone in a Microchannel. |
| ジャーナル | Anal Sci |
| Abstract |
Exosomes are of increasing research interest because they are integral to cell-cell communication and are implicated in various disease states. Here, we investigated the utility of using an ion-depletion zone in a microfluidic device to concentrate exosomes from the culture media of four types of cell lines. Furthermore, we evaluated the extent of damage to the exosomes following concentration by an ion-depletion zone microchannel device compared with exosomes concentrated by a conventional ultra-centrifugation technique. Our results conclusively demonstrate that significantly less damage is incurred by exosomes following passage through and concentration by the ion-depleted zone microchannel device compared to concentration by ultra-centrifugation. Our findings will help extend the utility of exosomes to various applications. |
| 巻・号 | 34(8) |
| ページ | 875-880 |
| 公開日 | 2018-1-1 |
| DOI | 10.2116/analsci.17P462 |
| PMID | 30101880 |
| MeSH | Cell Communication Cell Line Centrifugation Culture Media / chemistry* Exosomes / chemistry* Humans Ions / chemistry Microfluidic Analytical Techniques* |
| IF | 2.049 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MCF7(RCB1904) 293(RCB1637) A549(RCB0098) |