論文 - 詳細
| RRC ID | 54133 |
|---|---|
| 著者 | Matsuura R, Kaji H, Tomioka A, Sato T, Narimatsu H, Moriwaki Y, Misawa H, Imai K, Tsuji S. |
| タイトル | Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1. |
| ジャーナル | Sci Rep |
| Abstract |
The anti-mesothelioma mAb SKM9-2 recognizes the sialylated protein HEG homolog 1 (HEG1). HEG1 is a 400 kDa mucin-like membrane protein found on mesothelioma. SKM9-2 can detect mesothelioma more specifically and sensitively than other antibodies against current mesothelioma markers; therefore, SKM9-2 would be likely useful for the precise detection and diagnosis of malignant mesothelioma. In the present study, we investigated the epitope of SKM9-2. We analyzed the binding of SKM9-2 to truncated HEG1 and candidate epitope-fused glycosylphosphatidylinositol-anchor proteins. The epitope of SKM9-2 was identified as an O-glycosylated region, 893-SKSPSLVSLPT-903, in HEG1. An alanine scanning assay of the epitope showed that SKM9-2 bound to a simple epitope in HEG1, and the SKxPSxVS sequence within the epitope was essential for SKM9-2 recognition. Mass spectrometry analysis and lectin binding analysis of soluble epitope peptides indicated that the SKM9-2 epitope, in which Ser897 was not glycosylated, contained two disialylated core 1 O-linked glycan-modified serine residues, Ser893 and Ser900. Neuraminidase treatment analysis also confirmed that the epitope in mesothelioma cells contained a similar glycan modification. The specific detection of mesothelioma with SKM9-2 can thus be performed by the recognition of sialylated glycan modification in the specific region of HEG1. |
| 巻・号 | 8(1) |
| ページ | 14251 |
| 公開日 | 2018-9-24 |
| DOI | 10.1038/s41598-018-32534-8 |
| PII | 10.1038/s41598-018-32534-8 |
| PMID | 30250045 |
| PMC | PMC6155162 |
| MeSH | Antibodies, Monoclonal / genetics Antibodies, Monoclonal / immunology* Cell Line, Tumor Enzyme-Linked Immunosorbent Assay Epitopes / genetics* Epitopes / immunology Glycosylation Humans Lectins / chemistry Lectins / immunology Lung Neoplasms / genetics* Lung Neoplasms / immunology Lung Neoplasms / pathology Mass Spectrometry Membrane Proteins / genetics* Membrane Proteins / immunology Mesothelioma / genetics* Mesothelioma / immunology Mesothelioma / pathology Mesothelioma, Malignant N-Acetylneuraminic Acid / metabolism Peptides / genetics Peptides / immunology Polysaccharides / genetics Polysaccharides / metabolism Protein Binding / genetics Protein Binding / immunology |
| IF | 3.998 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-1(RCB2292) ACC-MESO-4(RCB2293) 293T(RCB2202) |