RRC ID 54176
Author Murata A, Hikosaka M, Yoshino M, Zhou L, Hayashi SI.
Title Kit-independent mast cell adhesion mediated by Notch.
Journal Int Immunol
Abstract Kit/CD117 plays a crucial role in the cell-cell and cell-matrix adhesion of mammalian mast cells (MCs); however, it is unclear whether other adhesion molecule(s) perform important roles in the adhesion of MCs. In the present study, we show a novel Kit-independent adhesion mechanism of mouse cultured MCs mediated by Notch family members. On stromal cells transduced with each Notch ligand gene, Kit and its signaling become dispensable for the entire adhesion process of MCs from tethering to spreading. The Notch-mediated spreading of adherent MCs involves the activation of signaling via phosphatidylinositol 3-kinases and mitogen-activated protein kinases, similar to Kit-mediated spreading. Despite the activation of the same signaling pathways, while Kit supports the adhesion and survival of MCs, Notch only supports adhesion. Thus, Notch family members are specialized adhesion molecules for MCs that effectively replace the adhesion function of Kit in order to support the interaction of MCs with the surrounding cellular microenvironments.
Volume 31(2)
Pages 69-79
Published 2019-2-15
DOI 10.1093/intimm/dxy067
PII 5123694
PMID 30299470
PMC PMC8562337
MeSH Animals Cell Adhesion / immunology Cells, Cultured Mast Cells / cytology Mast Cells / immunology* Mice Mice, Inbred C57BL Proto-Oncogene Proteins c-kit / immunology* Receptors, Notch / immunology*
IF 3.519
Times Cited 0
Mice RBRC01888