RRC ID 54197
著者 Ressurreição M, Warrington S, Strutt D.
タイトル Rapid Disruption of Dishevelled Activity Uncovers an Intercellular Role in Maintenance of Prickle in Core Planar Polarity Protein Complexes.
ジャーナル Cell Rep
Abstract Planar polarity, the coordinated polarization of cells in the plane of a tissue, is important for normal tissue development and function. Proteins of the core planar polarity pathway become asymmetrically localized at the junctions between cells to form intercellular complexes that coordinate planar polarity between cell neighbors. Here, we combine tools to rapidly disrupt the activity of the core planar polarity protein Dishevelled, with quantitative measurements of protein dynamics and levels, and mosaic analysis, to investigate Dishevelled function in maintenance of planar polarity. We provide mechanistic insight into the hierarchical relationship of Dishevelled with other members of the core planar polarity complex. Notably, we show that removal of Dishevelled in one cell causes rapid release of Prickle into the cytoplasm in the neighboring cell. This release of Prickle generates a self-propagating wave of planar polarity complex destabilization across the tissue. Thus, Dishevelled actively maintains complex integrity across intercellular junctions.
巻・号 25(6)
ページ 1415-1424.e6
公開日 2018-11-6
DOI 10.1016/j.celrep.2018.10.039
PII S2211-1247(18)31615-2
PMID 30403998
PMC PMC6231328
MeSH Animals Cell Polarity* DNA-Binding Proteins / metabolism* Dishevelled Proteins / metabolism* Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism* Dynamins / metabolism Epithelium / metabolism LIM Domain Proteins / metabolism* Multiprotein Complexes / metabolism* Protein Stability Protein Transport Pupa / cytology Pupa / metabolism Wings, Animal / cytology Wings, Animal / metabolism
IF 8.109
引用数 1
リソース情報
ショウジョウバエ 18361R-2