RRC ID 542
Author Kamita SG, Nagasaka K, Chua JW, Shimada T, Mita K, Kobayashi M, Maeda S, Hammock BD.
Title A baculovirus-encoded protein tyrosine phosphatase gene induces enhanced locomotory activity in a lepidopteran host.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Enhanced locomotory activity (ELA), such as wandering, is a normal behavior that occurs at the end of the larval stage in lepidopteran (butterflies and moths) insects. Baculovirus infection can also induce ELA in lepidopteran larvae. The belief is that the virus induces this behavior to increase its transmission [Goulson, D. (1997) Oecologia 109, 219-228]. Here we show that a baculovirus-encoded protein tyrosine phosphatase (PTP) gene (ptp) induces ELA that is activated by light. ELA was induced in silkworm Bombyx mori infected with the baculovirus B. mori nucleopolyhedrovirus (BmNPV) beginning at approximately 3.75 days postinfection (p.i.) and continued until 4.75 days p.i. The intensity of the ELA was dramatically reduced immediately before death at 5.25 days p.i. Light activated the intensity of the ELA by approximately 3-fold, and larvae with ELA showed positive phototropism. ELA was not induced in larvae of B. mori infected with a BmNPV ptp knockout mutant (BmPTPD). However, when a silkworm-derived ptp gene (Bmptp-h) was inserted into BmPTPD, ELA was partially recovered. Bmptp-h was identified from silkworms at 2 days after the start of the natural wandering stage. The deduced amino acid sequence of Bmptp-h showed 48.2% identify (80.7% similarity) to the deduced amino acid sequence of BmNPV ptp. On the basis of the high homology and larval stage at which Bmptp-h was isolated, we postulate that the modern baculovirus may have acquired its ptp gene from an ancestral host and that this gene was selectively maintained because it increases virus transmission.
Volume 102(7)
Pages 2584-9
Published 2005-2-15
DOI 10.1073/pnas.0409457102
PII 0409457102
PMID 15699333
PMC PMC548987
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Bombyx / enzymology Bombyx / genetics* Bombyx / physiology* Gene Deletion Genes, Insect Locomotion / genetics* Locomotion / physiology Molecular Sequence Data Mutagenesis, Insertional Nucleopolyhedrovirus / genetics Photobiology Protein Tyrosine Phosphatases / genetics* Protein Tyrosine Phosphatases / physiology Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Homology, Amino Acid Transfection
IF 9.504
Times Cited 62
WOS Category MULTIDISCIPLINARY SCIENCES
Resource
Silkworms EST database