論文 - 詳細
| RRC ID | 54298 |
|---|---|
| 著者 | Tanaka C, Itoh T, Iwasaki Y, Mizuno N, Nasuda S, Murai K. |
| タイトル | Direct interaction between VRN1 protein and the promoter region of the wheat FT gene. |
| ジャーナル | Genes Genet Syst |
| Abstract |
The wheat florigen gene Wheat FLOWERING LOCUS T (WFT, which is identical to VRN3) is an integrator of the vernalization, photoperiod and autonomous pathways in wheat flowering. Many studies have indicated that VERNALIZATION 1 (VRN1) directly or indirectly up-regulates WFT expression in leaves. VRN1 encodes an APETALA1/FRUITFULL-like MADS box transcription factor that is up-regulated by vernalization and aging, leading to promotion of flowering. In this study, the VRN1 protein was expressed as a His-Tag fusion protein in Escherichia coli and used in an electrophoretic mobility shift assay (EMSA). The results from the EMSA indicated that the VRN1 protein directly binds to the CArG-box in the promoter region of WFT, suggesting the direct up-regulation of WFT by VRN1 in the leaves of wheat plants. |
| 巻・号 | 93(1) |
| ページ | 25-29 |
| 公開日 | 2018-7-13 |
| DOI | 10.1266/ggs.17-00041 |
| PMID | 29343669 |
| MeSH | Cloning, Molecular Gene Expression Regulation, Plant MADS Domain Proteins / metabolism* Plant Leaves / genetics Plant Leaves / metabolism Plant Proteins / genetics* Plant Proteins / metabolism Promoter Regions, Genetic Protein Binding Triticum / genetics Triticum / metabolism* Up-Regulation* |
| IF | 0.917 |
| 引用数 | 5 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| コムギ | |