RRC ID 54302
著者 Stratton JA, Holmes A, Rosin NL, Sinha S, Vohra M, Burma NE, Trang T, Midha R, Biernaskie J.
タイトル Macrophages Regulate Schwann Cell Maturation after Nerve Injury.
ジャーナル Cell Rep
Abstract Pro-regenerative macrophages are well known for their role in promoting tissue repair; however, their specific roles in promoting regeneration of the injured nerve are not well defined. Specifically, how macrophages interact with Schwann cells following injury during remyelination has been largely unexplored. We demonstrate that after injury, including in humans, macrophages function to clear debris and persist within the nerve microenvironment. Macrophage ablation immediately preceding remyelination results in an increase in immature Schwann cell density, a reduction in remyelination, and long-term deficits in conduction velocity. Targeted RNA-seq of macrophages from injured nerve identified Gas6 as one of several candidate factors involved in regulating Schwann cell dynamics. Functional studies show that the absence of Gas6 within monocyte lineage cells impairs Schwann cell remyelination within the injured nerve. These results demonstrate a role for macrophages in regulating Schwann cell function during nerve regeneration and highlight a molecular mechanism by which this occurs.
巻・号 24(10)
ページ 2561-2572.e6
公開日 2018-9-4
DOI 10.1016/j.celrep.2018.08.004
PII S2211-1247(18)31245-2
PMID 30184491
MeSH Animals Blotting, Western Brain / cytology Brain / metabolism Cell Line, Tumor Cell Nucleus / metabolism Cell Survival / genetics Cell Survival / physiology* Cytoplasm / metabolism Female HeLa Cells Humans Macrophages / metabolism* Mice Nerve Regeneration / physiology Pregnancy RNA Polymerase II / genetics RNA Polymerase II / metabolism RNA, Messenger / metabolism Schwann Cells / cytology Schwann Cells / metabolism
IF 8.109
引用数 25
リソース情報
ラット SD-Tg(CAG-EGFP)4Osb (strainID=559)