RRC ID |
54302
|
著者 |
Stratton JA, Holmes A, Rosin NL, Sinha S, Vohra M, Burma NE, Trang T, Midha R, Biernaskie J.
|
タイトル |
Macrophages Regulate Schwann Cell Maturation after Nerve Injury.
|
ジャーナル |
Cell Rep
|
Abstract |
Pro-regenerative macrophages are well known for their role in promoting tissue repair; however, their specific roles in promoting regeneration of the injured nerve are not well defined. Specifically, how macrophages interact with Schwann cells following injury during remyelination has been largely unexplored. We demonstrate that after injury, including in humans, macrophages function to clear debris and persist within the nerve microenvironment. Macrophage ablation immediately preceding remyelination results in an increase in immature Schwann cell density, a reduction in remyelination, and long-term deficits in conduction velocity. Targeted RNA-seq of macrophages from injured nerve identified Gas6 as one of several candidate factors involved in regulating Schwann cell dynamics. Functional studies show that the absence of Gas6 within monocyte lineage cells impairs Schwann cell remyelination within the injured nerve. These results demonstrate a role for macrophages in regulating Schwann cell function during nerve regeneration and highlight a molecular mechanism by which this occurs.
|
巻・号 |
24(10)
|
ページ |
2561-2572.e6
|
公開日 |
2018-9-4
|
DOI |
10.1016/j.celrep.2018.08.004
|
PII |
S2211-1247(18)31245-2
|
PMID |
30184491
|
MeSH |
Animals
Blotting, Western
Brain / cytology
Brain / metabolism
Cell Line, Tumor
Cell Nucleus / metabolism
Cell Survival / genetics
Cell Survival / physiology*
Cytoplasm / metabolism
Female
HeLa Cells
Humans
Macrophages / metabolism*
Mice
Nerve Regeneration / physiology
Pregnancy
RNA Polymerase II / genetics
RNA Polymerase II / metabolism
RNA, Messenger / metabolism
Schwann Cells / cytology
Schwann Cells / metabolism
|
IF |
8.109
|
引用数 |
25
|
リソース情報 |
ラット |
SD-Tg(CAG-EGFP)4Osb (strainID=559) |