RRC ID 5431
Author Venkata Ramana V, Anil Kumar P, Srinivas TN, Sasikala Ch, Ramana ChV.
Title Rhodobacter aestuarii sp. nov., a phototrophic alphaproteobacterium isolated from an estuarine environment.
Journal Int J Syst Evol Microbiol
Abstract An ovoid to rod-shaped, phototrophic, purple non-sulfur bacterium was isolated from a brown-coloured microbial mat from the brackish water of Bhitarkanika mangrove forest, Dangmal, Orissa, India. Cells of strain JA296(T) were Gram-negative and motile, forming chains of four to eight cells. The colour of the cell suspension grown under anaerobic conditions in the light was yellowish green. Bacteriochlorophyll a and the carotenoids spheroidene and spheroidenone of the spirilloxanthin series were present as photosynthetic pigments. The bacterium was a facultative anaerobe and was able to grow photo-organoheterotrophically and chemo-organoheterotrophically. Thiamine was required as a growth factor. C(18 : 1)omega7c was the dominant fatty acid. Internal cytoplasmic membranes were of the vesicular type. Strain JA296(T) did not require NaCl for growth. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA296(T) was most closely related to Rhodobacter capsulatus ATCC 11166(T) (95.5 % sequence similarity) and clustered with species of the genus Rhodobacter of the family Rhodobacteraceae, class Alphaproteobacteria. On the basis of 16S rRNA gene sequence analysis and morphological and physiological characteristics, strain JA296(T) represents a novel species of the genus Rhodobacter, for which the name Rhodobacter aestuarii sp. nov. is proposed; the type strain is JA296(T) (=JCM 14887(T) =CCUG 55130(T)).
Volume 59(Pt 5)
Pages 1133-6
Published 2009-5-1
DOI 10.1099/ijs.0.004507-0
PII 59/5/1133
PMID 19406806
MeSH Bacterial Typing Techniques Bacteriochlorophyll A / metabolism Carotenoids / metabolism DNA, Bacterial / analysis Fatty Acids / analysis Fresh Water / microbiology* India Molecular Sequence Data Phenotype Phototrophic Processes* Phylogeny RNA, Ribosomal, 16S / genetics Rhizophoraceae Rhodobacter / classification* Rhodobacter / genetics Rhodobacter / isolation & purification Rhodobacter / physiology Sequence Analysis, DNA Species Specificity Trees
IF 2.415
Times Cited 14
Resource
General Microbes JCM14887